胶质母细胞瘤分子亚型的功能磁共振影像基因组学研究

项目名称： 胶质母细胞瘤分子亚型的功能磁共振影像基因组学研究

项目编号： No.81471652

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 张辉

作者单位： 山西医科大学

项目金额： 80万元

中文摘要： 胶质母细胞瘤(GBM)是成人颅脑最常见的原发性恶性肿瘤，病理级别相同的GBM预后及对放化疗反应不同，这与GBM分子亚型有关。功能磁共振可获得比常规磁共振更多组织微观结构和功能信息。我们前期研究结果证实，磁共振征象与星形细胞瘤分级及恶性度关系密切，表明磁共振征象可以判断GBM恶性生物学行为。本课题在前期工作基础上，拟采用高通量微小RNA测序技术，构建GBM分子亚型；应用近年提出的影像基因组学（radiogenomics）新方法，整合微小RNA和功能磁共振技术，建立GBM不同分子亚型微小RNA与功能磁共振定量指标的关系，获得GBM不同亚型功能磁共振特征指标及阈值，探讨功能磁共振影像学特征所包含的生物学意义；应用前瞻性研究方法，验证功能磁共振评估GBM分子亚型的准确性。研究成果将为应用非侵袭性磁共振影像学预判肿瘤基因分型提供新的思路和依据，为个体化治疗方案的制定提供无创可行的技术手段。

中文关键词： 胶质母细胞瘤；分子亚型；功能磁共振成像；微小RNA；影像基因组学

英文摘要： Glioblastoma (GBM) is the most common malignant brain tumor in adults, Affected patients with the same pathological grade have a very different prognosis and responses to radiotherapy and chemotherapy，which is related to the GBM molecular subtypes. Functional MRI can obtain more microstructural and functional information of tissues compared with the conventional MRI. In previous study we confirmed that MRI features were closely associated with the grading and malignant degree of astrocytoma, which showed that MRI features could determine the malignant biological behavior of GBM. Based on the previous work, this study wants to establish GBM molecular subtypes by using high-throughput miRNA sequencing technique；applying the new method of radiogenomics proposed in recent years, which integrating of miRNA sequencing and functional MRI techniques to establish the relationship between miRNA of GBM different molecular subtypes and quantitative parameter of functional MRI, thus obtaining the characteristic parameter and threshold of functional MRI in GBM different subtypes, and investigating the biological significance contained in functional MRI features；then，using prospective research to validate accuracy of functional MRI in assessing GBM molecular subtypes. The results of the study will provide new ideas for noninvasively estimating tumor genotyping by MRI, and also provide feasible noninvasive techniques for making individualized treatment.

英文关键词： glioblastoma;molecular subtypes;founctionalMRI;microRNA;radiogenomic