基于纳米孔膜的微纳机电系统及其在食源性病原微生物检测上的应用

项目名称： 基于纳米孔膜的微纳机电系统及其在食源性病原微生物检测上的应用

项目编号： No.81471747

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 杨莫

作者单位： 香港理工大学深圳研究院

项目金额： 73万元

中文摘要： 近年来，食源性病原微生物引起的疾病严重威胁着人类的健康和生命安全，日益成为一个重要的公共卫生问题，而现有的病原微生物检测方法，操作复杂、费时费力、灵敏度不高，每次只能检测一种或少量几种病原体，无法对同一来源的多种病原体进行同时检测。本项目将采用高灵敏性的氧化铝纳米孔膜电阻抗传感器，以体外合成的病原微生物适体分子作为识别元件，结合具有高通量特性的阵列化微流控芯片技术，通过对纳米孔膜适体传感器的电阻抗特性、传感响应机理及传感特性的研究，以期构建出基于纳米孔膜的生物微纳机电系统传感芯片用于多种病原微生物的同时、快速及高灵敏度的检测。项目立足于纳米孔膜传感器的灵敏性，并结合适体的特异性和稳定性，提供了一个新的具有高度特异性和检测灵敏度的传感机制，构建出的生物微纳机电系统检测芯片，为病原微生物的检测研究提供了一个新的传感技术平台，在食品安全和环境监测上有着重要的应用。

中文关键词： 生物微机电系统；纳米传感器；纳米孔膜；生物传感器；微流控芯片

英文摘要： In recent years, the diseases caused by foodborne pathogenic microorganisms increasingly threaten the health and safety of people and have emerged as major concerns of public. However, the current detection methods for pathogenic microorganisms are labor-intensive, time-consuming and have relatively low sensitivity with complicated operation procedures. Moreover, these methods can only detect one or few types of pathogenic microorganism for each testing and are not available for simultaneous detection of multiple types in a single sample. The purpose of this project is to develop a Biomedical Nano-Electro-Mechanical-System (BioNEMS) based biosensing platform integrated with aptamer-functionalized nanoporous alumina membrane which enables fast, sensitive and high-throughput analytical methods for simultaneous detection of multiple foodborne pathogenic microorganisms. The combination of chemically synthesized aptamer molecules as recognition elements with high specificity and stability, nanoporous membrane based electrochemical sensor with high sensitivity, and microfluidic chip for high-throughput analysis capability, provides a novel biosensing platform for foodborne pathogenic microorganism detection. The results of the proposed work are expected to advance the research for exploring nanoporous materials for biosensing application and developing a new hybrid BioNEMS based biosensing platform for foodborne pathogenic microorganism detection. Successful delivery of the proposed milestones will have a wide range of applications from food safety testing to environmental monitoring.

英文关键词： BioMEMS;Nanosensor;Nanporous membrane;Biosensor;Microfluidic chip