用于水质生化需氧量检测的微生物膜反应器的构建、表征及应用研究

项目名称： 用于水质生化需氧量检测的微生物膜反应器的构建、表征及应用研究

项目编号： No.21305132

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 刘长宇

作者单位： 中国科学院长春应用化学研究所

项目金额： 25万元

中文摘要： 利用微生物传感技术检测污水中可生物降解有机物含量的方法往往因微生物的种类及数量有限，导致有机物的选择性、低效降解。微生物膜反应器具有多菌群组成、高单位密度的物理特性，是理想的微生物降解污水有机物的生物识别元件，是快速生化需氧量（BOD）检测的核心构件。本项目将系统地研究管状微生物膜反应器的构建及表征方法，并提出BOD检测的新原理和新方法。创新性地以污水内源微生物为培养微生物膜反应器的微生物源，以碳基材料功能化的管路为基底，构建流通式微生物膜反应器。通过本项目的研究，要掌握内源微生物在功能化的反应器基底上成膜关键性条件，实现三维、多尺度下微生物膜结构的微观表征，揭示微生物膜的代谢动力学特性及不同时期下的细胞微观状态。本项目研究可获取微生物膜反应器制备的关键技术，并为发展高稳定、超耐用、少维护和低消耗的快速BOD在线监测方法提供科学依据。

中文关键词： 微生物膜；反应器；流通式；在线；生化需氧量

英文摘要： The microbial sensing technology was commonly used for detecting biodegradable organic compounds in wastewater, however, resulted a selective, low efficiency biodegradation due to the microbial species and quantities. The biofilm reactor consists of multi-species microorganisms with high unit density, which is an ideal biological recognition element in biodegrading organic compounds in wastewater. The biofilm reactor is also a key component in rapid biochemical oxygen demand (BOD) determination. This project will study the tubular biofilm reactor cultivation method systematically, and propose new BOD determination principles and methods. Innovatively, endogenous microbial species in wastewater were used as microbial seeds in cultivation the biofilm reactor, coupled with carbon based material modified tubes as substrate in fabricating the plug flow tubular biofilm reactor. In this project, the key biofilm formation conditions on the functionalized substrate will be obtained, as well as the three-dimensional, multi scale microcosmic characterization of the biofilm structure. The metabolic dynamic characteristics and cellular microcosmic conditions in multi periods will also been revealed. This project will obtain the key technologies in preparing biofilm reactor, and afford scientific foundations in the developing

英文关键词： biofilm；reactor；plug flow；online；BOD