稻瘟病菌G蛋白调控因子MoRgs7的结构域及其互作蛋白的功能分析

项目名称： 稻瘟病菌G蛋白调控因子MoRgs7的结构域及其互作蛋白的功能分析

项目编号： No.31201471

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 植物保护学、园艺学与植物营养学

项目作者： 张海峰

作者单位： 南京农业大学

项目金额： 24万元

中文摘要： 异三聚体G蛋白信号途径是真核细胞感受外源信号，并将外源信号整合到胞内信号系统中最重要的信号传导途径之一，G蛋白调控因子RGS是该信号途径的负调控因子。项目组前期研究发现稻瘟病菌含有8个RGS:调控该病菌的生长发育及对水稻的致病性，其中参与致病过程的MoRgs7预测含有信号肽、7次跨膜的GPCR结构域和RGS 结构域。本项目拟阐明MoRgs7的三个结构域在该病菌生长发育和致病中的作用；采用酵母双杂交和co-IP 技术鉴定和验证MoRgs7的互作蛋白，并解析其生物学功能，从而阐明MoRgs7调控的信号途径在该病菌生长发育和致病中的作用机制。研究结果不仅有助于深入揭示G蛋白信号途径介导的分子机制，而且可为开发以MoRgs7互作蛋白为靶标的高效、低毒的杀菌剂提供参考。

中文关键词： 稻瘟病菌；G蛋白调控因子；互作蛋白；调控蛋白；调控机制

英文摘要： Signal transduction cascades are the primary means by which external cues are communicated to the nuclei of eukaryotic organisms including fungi. Heterotrimeric guanine-nucleotide binding protein (G-protein) signaling is one of the most important mechanisms by which eukaryotic cells sense extracellular signals and integrate them into intrinsic signal transduction pathways. Regulators of G-protein signaling (RGS) proteins contain a conserved domain of 120 amino acids that are required for activity and function as key negative regulators of G-protein signaling pathways. Our previous study revealed that eight RGS proteins play important roles in growth, development and pathogenicity of Magnaporthe oryzae. MoRgs7, one of RGS proteins which containing a signal peptide, a GPCR(7 transmembranes)domain as well as a RGS domain, is required for full virulence in this phytopathogen. Therefore, function analysis the domains of MoRgs7 and identify its binding proteins will help us further understand the molecular mechanism of G protein signaling pathway in rice blat disease. In this study, we will construct the vectors expression different domains of MoRgs7 and transferred to the ?Morgs7 mutant, respectively. The biological phenotypes of the resulting transformants will be analysed in comparison with wild type Guy11 and the

英文关键词： Magnaporthe oryzae；Regulators of G-protein signaling；binding protein；regulatory protein；regulatory mechanism