低温氩氧等离子体活化表面对种植体骨结合的影响及其机制研究

项目名称： 低温氩氧等离子体活化表面对种植体骨结合的影响及其机制研究

项目编号： No.81500882

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 柏娜

作者单位： 青岛大学

项目金额： 18万元

中文摘要： 种植义齿已成为牙列缺损和牙列缺失的常规修复方法，其成功的关键在于骨结合，而骨结合的关键在于“骨生长”，即种植体周围新骨的生成。种植体表面生物活性影响骨结合进程。实验证明钛种植体表面即使经过各个表面处理后短时间内具有生物活性，但在空气中暴露之后会形成一层保护层，即发生生物老化，生物活性将大大降低。如何在种植体植入前即时的活化种植体表面并使其保持原来较高的生物学活性是近年来口腔种植领域研究的新热点，其中低温等离子体活化技术以其高效率、低能耗、安全和无二次污等优势越来越被研究者们所青睐。本实验旨在以Ar+5%O2的混合气体为输入气体产生低温等离子体，对种植体表面进行活化处理，通过外科手术方法植入大鼠股骨内，评价种植体周围的骨结合情况；并在体外进行材料与成骨细胞粘附共培养，通过观察粘附、增殖、分化等生物学行为探讨低温等离子体活化材料后的促骨结合机制，为低温等离子体对种植体表面材料活化的新途径提供实验依据。

中文关键词： 低温等离子体；种植体；表面处理；骨结合；成骨细胞

英文摘要： Implant denture has become one of the conventional repairment methods for tooth defect and edentulous dentition. The key to success of dental implant lies in physical osseointegration between bone tissue and implant; while the key of osseointegration lies in bone growth which was new bone formation around the implant and was response of the implant to the host. The low process of osseointegration was related to the biological aging of the implant surface, which reduced the influence of the biological response of the peri-implant tissues to the implant. How to immediately active the implant surface before implant is inset into bone and keep the original high biological activity is the new hot spot in the research field of Oral Implantology recently. The outstanding characteristics of using Cold, Atmospheric Pressure Plasma such as high efficiency, low energy consumption, safety, no secondary pollution, simple, and no expensive vacuum equipment and complex time-consuming procedures needed under the effect of atmospheric pressure is possiblely good for developing one fast, easy, and secure new implant surface activation technique just before oral implant surgery. This study intends to produce Cold, Atmospheric Pressure Plasma by mixture with Ar +5% O2, which was used to active the surface coated by implant titanium. Rat femur implant models were established, and titanium and HA-coated implants activated by Cold, Ar+5%O2 Plasma were implanted using self-control experimental approach to evaluate the implant osseointegration rate. Rat osteoblasts from skull were cultured and purified, then the osteoblasts were inoculated and cultivated on different titanium plates activated by Cold, Ar+5%O2 Plasma. By observing the biological behavior, such as cell adhesion, proliferation and differentiation, we hope to reveal the the mechanism of effects of Cold, Ar-O2 Plasma activated surface on implant osseointegration.

英文关键词： Cold Plasma;implant;surface treatment;osseointegration;osteoblast