基于HIF-1a/VEGF信号通路探讨电针刺激对脊髓损伤神经再生微环境调控机制研究

项目名称： 基于HIF-1a/VEGF信号通路探讨电针刺激对脊髓损伤神经再生微环境调控机制研究

项目编号： No.81460746

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 周宾宾

作者单位： 广西中医药大学

项目金额： 50万元

中文摘要： 脊髓损伤(SCI) 后血氧微环境构建及内源性神经干细胞的激活是决定神经再生的关键因素，HIF-1a/VEGF是调控缺血缺氧的重要信号通路，但对其如何参与SCI血氧微环境重建机理尚不清楚。本课题组前期依据中医治萎独取阳明、调理气血的理论，使用电针治疗SCI取得满意临床疗效。据此提出了电针可通过干预HIF-1a/VEGF信号转导，改善脊髓血氧微环境，促进神经再生的假说，拟通过建立大鼠SCI模型，应用基因芯片等技术观察HIF-1a/VEGF信号通路对脊髓血氧微环境构建的调控作用；应用细胞病理及分子生物学相关技术，观察电针对脊髓功能修复、神经再生、HIF-1a/VEGF信号通路相关分子、炎症因子、内源神经生长因子、及凋亡相关分子表达及内源性神经干细胞的激活的影响，从而阐明电针调控HIF-1a/VEGF信号通路促进神经再生治疗脊髓损伤的机理。

中文关键词： 脊髓损伤；电针；神经再生；微环境；信号通路

英文摘要： To rebuild the oxygen microenvironment and activation of endogenous neural stem cell after spinal cord injury (SCI) are key factors in promoting nerve regeneration. HIF-1α/VEGF is the central signaling pathway in regulation of ischemia and hypoxia, but the mechanism for oxygen microenvironment reconstruction is still poorly understood after SCI. Our group preliminary use electroacupuncture to treat SCI based on the theory of the rule a trophy was given the Yangming and reconcile Qi and blood of traditional Chinese medicine (TCM), and got an excellent clinical efficacy. Therefore, we speculate electroacupuncture could improve spinal cord blood oxygen microenvironment to promote nerve regeneration by regulating HIF-1α/VEGF signaling pathway . In order to clarify this, At first,we technology to observed functional repair of nerve, the promoting nerve regeneration in the spinal cord injury in rats, to clarify the mechanisms of electroacupuncture to promote nerve regeneration based on by regulating HIF-1α/VEGF signaling pathway, then apply Cell pathology and molecular biological technology to analyze related rembers of HIF-1α/VEGF signal pathway, and to speculate the mechanisms of its regulating oxygen microenvironment.To detect HIF-1α/VEGF signaling pathways associated protein after electroacupuncture，and to observe functional repair of nerve, the promoting nerve regeneration in the spinal cord injury in rats, to clarify the mechanisms of electroacupuncture to promote nerve regeneration based on by regulating HIF-1α/VEGF signaling pathway and inflammatory cytokines,nerve growth factors，apoptosis-related molecule and activation of endogenous neural stem cell which are important to further explore more effective electroacupuncture treatment program for SCI.

英文关键词： spinal cord injury;electroacupuncture;nerve regeneration;spinal inmicroenvironment;signal channel