FcγRIIIA介导冠心病血瘀证的发病机制及川芎赤芍有效部位的干预效应研究

项目名称： FcγRIIIA介导冠心病血瘀证的发病机制及川芎赤芍有效部位的干预效应研究

项目编号： No.81202837

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 马晓娟

作者单位： 中国中医科学院西苑医院

项目金额： 23万元

中文摘要： 课题组前期利用全基因组芯片技术，构建了冠心病血瘀证差异基因表达谱，从核酸水平揭示了冠心病血瘀证与炎症免疫反应的相关性，并对筛选出的目标基因（包括FcγRIIIA）进行了初步验证。并通过Signal-Net信号转导网络的构建，分析发现FcγRIIIA在冠心病血瘀证疾病过程中具有调控作用。 本研究在对目标分子FcγRIIIA进行临床相关性验证及来源鉴定的基础上，通过构建人外周血单核细胞-内皮细胞实验体系，探讨目标分子FcγRIIIA在单核-内皮细胞黏附中的作用，并通过构建apoE基因敲除小鼠动脉粥样硬化（AS）模型，观察FcγRIIIA表达对AS斑块稳定性以及炎症因子表达的影响；并通过观察中药的干预效应，评价活血化瘀中药川芎赤芍有效部位抗内皮损伤、减轻炎症反应、稳定斑块等抗动脉粥样硬化作用，及目标分子FcγRIIIA所扮演的"角色"，探讨活血化瘀中药抗动脉粥样硬化的作用机制及可能的作用靶点。

中文关键词： 冠心病；血瘀证；动脉粥样硬化；川芎赤芍有效部位；

英文摘要： In previous study, we constructed the differential gene expression profile of coronary heart disease (CHD) with blood stasis syndrome by oligonucleotide gene microarray, which findings suggested the relevance of inflammation and immune responses to CHD with blood stasis syndrome at DNA level. These screened target genes were verificated by real-time reverse transcription polymerase chain reaction, including FcγRIIIA. Moreover, the regulatory role of FcγRIIIA was found in CHD with blood stasis syndrome by signal-net signal transduction network. In the present study, we will carry out the clinical correlation identification and source verification of target molecule FcγRIIIA. Basing on the results of validation, we will construct the cell system of human peripheral blood mononuclear cell-endothelial cell to explore the effect of the target molecule FcγRIIIA in monocyte-endothelial cell adhesion. Besides, we will make atherosclerosis model in apoE-knockout mouse to observe the effect of FcγRIIIA in atherosclerosis plaque stability and inflammatory cytokines expression. The chuanxiong and paeoniae radix effective parts was administrated in cell and mouse experiments to evaluate the effcts of traditonal medicine with promoting blood and removing stasis in anti-endothelial damage, lessening of inflammation and stabliz

英文关键词： coronary heart disease；blood stasis syndrome；artherosclerosis；chuanxiong and paeoniae radix effective parts；