Syk作为肝癌复发转移标志物的研究

项目名称： Syk作为肝癌复发转移标志物的研究

项目编号： No.30872489

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 轻工业、手工业

项目作者： 元云飞

作者单位： 中山大学

项目金额： 30万元

中文摘要： 肝癌Syk基因的研究文献罕见报道。在前一个国家自然科学基金项目《Syk基因与肝细胞癌侵袭性的研究》等资助下，研究发现：Syk两种异构体Syk(L)和Syk(S)均同肝癌预后有一定关系；Syk(L)可能在转录水平下调癌基因Cyclin D1的表达；Syk(S)主要在肝肿瘤组织中表达，在正常无肝硬化肝组织中不表达。本研究拟构建Syk(L)和Syk(S)被上调/下调的肝癌细胞模型，观察细胞表型的变化和裸鼠体内成瘤、转移潜能的改变，Cyclin D1是否伴随改变；进一步研究Syk(L)和Syk(S)与Cyclin D1在肝癌组织中的表达规律，并结合临床病理和预后因素进行分析；前瞻性研究外周血中游离Syk(S)mRNA的表达水平与肝癌术后转移复发的关系。研究结果将有助于了解Syk两种异构体在肝癌中的功能和作用机制，为肝癌的分子分期和靶点治疗等提供新的理论依据。

中文关键词： 肝细胞癌；Syk；复发；分子标志物

英文摘要： Study on Syk gene in hepatocellular carcinoma(HCC) was rarely reported in literature. Under the support of NSFC for our previouse project:SYK GENE AND INVASIVENESS OF HEPATOCELLULAR CARCINOMA, we found that the two isoforms of Syk gene, Syk(L) and Syk(S) was correlated with prognosis of HCC patients after tumor resection. Syk(L) could downregulate the expression of Cyclin D1 in HCC. Syk(S) was mainly expressed in neoplastic tissues of HCC, but not in normal non-cirrhotic liver tiussues. In this project, the Syk expression will be up-regualated or down-regulated in HCC cell lines, and then the changes of phenotype and carcinogenesis or metastasis potentials of HCC cells will be explored. The changes of Cyclin D1 expression will also studied. The correlation of Syk(L),Syk(S) and Cyclin D1 expression in HCC with clinicopathological and prognostic factors will be further investigated. The correlation of Syk(S)mRNA expression in serum with HCC recurrence will be propectively studied. This study will help to understand the roles of two isoforms of Syk gene in carcinogenesis, molecular staging and target therapy for HCC.

英文关键词： HCC; Syk; Recurrence; Molecular marker