鳜鱼驯食性状相关基因功能分析与机理研究

项目名称： 鳜鱼驯食性状相关基因功能分析与机理研究

项目编号： No.31272641

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 梁旭方

作者单位： 华中农业大学

项目金额： 80万元

中文摘要： 鳜鱼驯食人工饲料是水产界世界级难题，具重要理论与应用价值。项目组在易驯食与不易驯食鳜鱼脑、肝等组织转录组及数字表达谱测序基础上，已筛选得到鳜鱼驯食性状相关基因，并已克隆获得其中9个摄食相关基因的基因组全序列，采用易与不易驯食鳜鱼各200尾通过CRS-PCR、PCR-SSCP、SB-ASA等方法对其SNP位点进行了鉴定与相关性分析，得到了4个鳜鱼驯食相关SNP位点。本项目拟进一步对其它21个涉及摄食调控通路、视网膜感光通路、昼夜节律通路、消化代谢通路、学习记忆通路的鳜鱼驯食性状相关基因SNP位点进行鉴定与分析，并采用细胞转染、同位素标记法、放射性免疫分析法、共聚焦成像、Real time-PCR、Western Blotting、免疫组化、酶活性测定等方法，重点研究这些基因的信号通路及作用途径。本项目从基因水平上探讨鳜鱼驯食性状的决定机制，将为基因辅助选育易驯食优良鳜鱼新品种、品系奠定基础。

中文关键词： 鳜鱼；驯食；基因；信号通路；调控机理

英文摘要： Mandarin Fish, an absolutely carnivorous fish, usually refuse to eat dead food or man-made feed due to its specific feeding habit. Under cultivation, mandarin fish only consume live prey fish throughout their life and refuse dead prey fish or artificial diets. This problem has been widely known as a hard nut to crack in the field of aquaculture in China. Our research group has obtained the transcriptome database of mandarin fish from the brain, liver and other tissues of two groups (200 individuals easy to domesticate and 200 individuals hard to domesticate). Based on the results of food habit domestication-related genes screened from the transcriptome database of mandarin fish, we have analyzed and identified the SNPs for the food habit domestication traits-related genes, and four SNPs have been found for the first time using methods including CRS-PCR, PCR-SSCP and SB-ASA. We will continue to explore the SNPs of the mandarin fish genes related to pathways of food appetite control, retinal photosensitivity, circadian rhythm, metabolism, learning and memory, in order to clarify the relation between gene expression and nucleotide polymorphism. In addition, in vitro cell system, isotope-tracing methods, confocal imaging, radioimmunoassay, real time-PCR, Western blotting, immunohistochemistry, enzyme assay and other

英文关键词： Mandarin fish；Food habit domestication；Gene；Signal pathway；Regulatory mechanism