拟南芥STRF1蛋白参与植物盐胁迫信号转导途径的机理研究

项目名称： 拟南芥STRF1蛋白参与植物盐胁迫信号转导途径的机理研究

项目编号： No.31300221

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 唐三元

作者单位： 中国科学院遗传与发育生物学研究所

项目金额： 23万元

中文摘要： 盐胁迫是影响植物生长发育并造成农作物大规模减产的主要原因之一，泛素介导的26S 蛋白酶体降解途径在植物参与盐胁迫信号转导中起着重要的作用。为探索未知泛素连接酶E3参与盐胁迫的机制，本研究对筛选到的E3耐盐突变体(strf1)进行深入研究。项目前期工作表明该蛋白（STRF1）参与了植物对盐胁迫的响应；其定位于质膜和内涵体上，调控了植物细胞的内吞过程。本项目已筛选到STRF1的相互作用蛋白IntP1，拟运用Pull-down、Co-IP等体外和体内实验分析STRF1和IntP1的相互关系，并通过对STRF1/IntP1复合体功能的研究来揭示STRF1参与植物盐胁迫响应的分子机理；由于STRF1参与了植物细胞的内吞过程，且内吞过程在植物应对盐胁迫中起着重要作用，本项目将探索STRF1蛋白是否是通过影响植物的膜运输来参与盐胁迫的信号转导途径；该相关研究将为农作物的改良提供理论依据。

中文关键词： STRF1；膜运输；盐胁迫；泛素化；

英文摘要： Salt stress is one of the major detrimental abiotic stresses that affect plant growth and development, consequently decreased the yield of crops. The ubiquitin/proteasome system has been demonstrated to play critical roles in salt stress response of plant. In order to study functions of some novel E3 ligases involved in salt stress signaling, we focus mainly on the mutant line of E3 (strf1), which is insensitive to salt stress through sceening the mutant pool. Our preliminary results indicated STRF1 was involved in salt stress response pathway; STRF1 was localized on the plasma membrane and endosomes；STRF1 affected endocytosis in plant. To deeply reveal STRF1's function in plant salt stress response, yeast two-hybrid was applied to sreen protiens interacting with STRF1. We had proved that IntP1 was the protien interacting with STRF1. We will confirm the interaction between STRF1 and IntP1 in vitro and in vivo through Pull-down, Co-immunoprecipitation (Co-IP) and bimolecular fluorescence complementation (BiFC) assays. The degradation of IntP1 through the 26S proteasome in the presence of STRF1 and the stability of IntP1 protein in strf1 mutant will be investigated. Furthermore, the phenotype of IntP1 overexpressing plants stressed by salt will be analyzed. The study might provide some clues to investigate the rol

英文关键词： STRF1；Membrane trafficking；salt stress；ubiquitination；