新疆维汉民族冠心病秽浊痰阻证基因组学分析及与地域因素交互作用研究

项目名称： 新疆维汉民族冠心病秽浊痰阻证基因组学分析及与地域因素交互作用研究

项目编号： No.81460675

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 王晓峰

作者单位： 新疆医科大学

项目金额： 47万元

中文摘要： 大量研究证实，因特殊地域因素及遗传的异质性等导致不同民族存在着不同的易感基因与遗传特点。新疆冠心病因其特殊的地域性因素（气候寒冷干燥、多民族聚集、不同的民俗及饮食习惯等）及不同民族的遗传特征，冠心病发病率高于全国且中医证型具地域特点。前期研究显示，秽浊痰阻证占新疆冠心病患者的46.7%。本项目以维、汉民族秽浊痰阻证与非秽浊痰阻证冠心病患者为研究对象，采用全基因组表达谱芯片，研究秽浊痰阻证的基因表达谱；基于基因数据库对差异基因进行功能和通路注释，构建共表达网络图。运用qRT-PCR对筛选出的差异基因进行芯片结果验证，并筛选出显著性差异基因，构建新疆冠心病秽浊痰阻证的的诊断模型。本研究以维、汉民族冠心病中医证型特征为基础，通过证候要素与基因组学及代谢组学的研究，寻找维、汉民族冠心病秽浊痰阻证生物学基础，揭示发病机制，为本地区冠心病的防治提供新的思路和方法。

中文关键词： 维；汉民族；冠心病；秽浊痰阻证；地域性因素；基因表达谱分析

英文摘要： Many study confirmed that there are characteristic differences among different ethnic population in disease-related predisposing genes due to genetic heterogeneity and special regional factors. Early studies show that the incidence of coronary heart disease(CHD) is higher in Xinjiang than that in other province, and the pattern of TCM syndrome of CHD present a special regional feature,which the Huizhuotanzu type is the leading TCM syndrome type(46.7%),result from such special regional factors as cold and arid Climate, multiethnic aggregation, various folk custom and diet Habits Our study aim to explore the genetic differences of TCM Syndrome (Huizhuotanzu type and Non-Huizhuotanzu type) between Uygur and Han population,using genomewide expression profile microarray chip analyzed the differential expression of predisposing genes of CHD in Uygur and Han population with a TCM syndrome type of Huizhuoitanzu, Construction coexpression network by functional remark the differential expression gene channel, validated the screening genes by qRT-PCR in order to achieved the candidate genes,then construction TCM diagnostic model of CHD with Huizhuotanzu type in Xinjiang population,. Our study based on the special regional characteristc of TCM sydrome type of CHD in Uygur and Han population,genetical analyzed the differencies in TCM Zhenhou essence between two population by genomic and metabonomics methods, explore the internal mechanism,demonstrate the contribution of special regional characteristic of patterns of TCM syndrome to the pathogenesis of CHD with Huizhuotanzu type, established an evidence-based strategy of prevention and treatment on coronary heart disease correspond to the special regional environment in Xinjiang region.

英文关键词： Uygur and Han nationality;Coronary heart disease;HuiZhuoTanZu Type;Special regional facters;Genomewide expression profile chip analyze