人工关节假体不同含硅涂层对成骨细胞活性及基因表达谱的研究

项目名称： 人工关节假体不同含硅涂层对成骨细胞活性及基因表达谱的研究

项目编号： No.81472060

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 孙俊英

作者单位： 苏州大学

项目金额： 80万元

中文摘要： 假体松动是人工关节置换术的常见并发症,提高人工关节假体与骨组织的骨性结合是避免这一并发症，确保该置换术获得成功的关键之一。研究表明将生物活性材料与假体改性技术相结合，可以提高成骨细胞的活性，加速并增强假体与骨组织的骨性愈合。近年来，我们在这一领域内，已相继采用了等离子喷涂、微弧氧化、以及阴极弧沉积等改性技术，成功的获得了关节假体表面的含硅涂层。本课题将比较经这三种不同涂层技术获得的含硅涂层，对成骨细胞黏附、增殖及分化等生物活性的影响，并采用基因芯片技术在较大范围内比较成骨细胞基因表达谱的差异，进而系统深入地阐释硅离子调节成骨细胞生物活性的基因机制及所涉及的信号转导通路。通过本课题研究，以期获得一种最优化的人工关节假体改性技术，为改进人工关节假体的表面涂层，提供一定的理论和应用基础。

中文关键词： 表面改性；硅；成骨诱导；生物活性；基因芯片

英文摘要： Prosthesis loosening is a common complication fo total joint arthroplasty. The improvement of interfacial osteointegration between the prosthesis and surrounding bone is a key factor for the long-term success of arthroplasty, which could effectively avoid loosening. Researches have shown that the combination of biomaterials and surface modification of prosthesis can significantly enhance the osteoblastic activity and accelerate healing between the implants and bone tissue. Recently, we have prepared prosthesis coating incorporating silicon by plasma spraying, micro-arc oxidation and cathodic arc plasma deposition. In this project, three samples incorporating Si will be prepared, including plasma sprayed dicalcium silicate coatings, porous silicon-incorporated titianium dioxide coatings and silicon-doped titanium dioxide films deposited by cathodic arc. The investigation on the biological responses of osteoblast cells cultured on the three samples, including adhesion, proliferation and differentiation. Gene expression profiling by DNA microarray is a new molecular technology that allows the analysis of gene expression in a cell system. The exact effect of Si ions on osteoblast cells will be evaluated by DNA microarray.The data may help to provide a better understanding of molecular mechanism of implants modified by Si ions and serve as a model for studying the other materials.

英文关键词： Modification;Silicon;Osteoblast;Bioactivity;Microarray