金属硫化物纳米粒子与明胶蛋白质的原位相互作用研究

项目名称： 金属硫化物纳米粒子与明胶蛋白质的原位相互作用研究

项目编号： No.21067001

项目类型： 地区科学基金项目

立项/批准年度： 2011

项目学科： 生物科学

项目作者： 唐世华

作者单位： 广西民族大学

项目金额： 12万元

中文摘要： 纳米颗粒与蛋白质间相互作用的研究，对其在生物体内的应用具有重要意义。本项目针对金属硫化物纳米粒子与明胶大分子原位相互作用开展了初步的探索。研究发现，金属离子与明胶大分子链上的活性基团通过配位或静电作用形成金属离子/明胶络合物，进而与硫离子形成金属硫化物，不仅对明胶蛋白质的内源荧光有着较强烈的猝灭作用，且引起明胶大分子结构的变化。根据Scatchard位点结合模型，推导出了用于计算结合常数和结合位点数的两个公式：明胶荧光猝灭与加入金属离子总浓度的关系式；体系吸光度和加入金属离子总浓度的关系。金属硫化物纳米粒子在明胶大分子上的生成服从准一级反应的动力学规律。已发表论文4篇，会议论文1篇。

中文关键词： 金属硫化物纳米粒子；明胶；相互作用；机制

英文摘要： Studies on the interaction of nanoparticles with protein are important for its application in living organisms. The interaction between the metal sulfide nanoparticles and gelatin are researched in this item. Preliminary findings indicated that there is a chemical bonding between the metal ions and the active groups (e.g. amide group, carboxy group and so on) of gelatin macromolecule, and then the sulfur ions reacted with metal ions to form the metal sulfide nanoparticles in gelatin matrix; which quenched significantly the intrinsic fluorescence of gelatin, and caused the conformation change of gelatin macromolecule. For calculating the binding constants and binding sites of the metal sulfide nanoparticles with gelatin, two linear equations were introduced on the basis of the relationship between the total concentration of metal ion, and the absorbance of system, the ?uorescence intensity of gelatin, respectively, according to Scatchard binding site model. The binding of metal sulfide nanoparticles onto gelatin properly correlate with the pseudo-first-order kinetic model. Our groups have published 4 scientific articles in journals and 1 meeting paper.

英文关键词： metal sulfide nanoparticle; gelatin; interaction; mechanism