量子点与Cu2+对L02细胞联合毒性的木马机理研究

项目名称： 量子点与Cu2+对L02细胞联合毒性的木马机理研究

项目编号： No.21307031

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 环境科学、安全科学

项目作者： 赵宇侠

作者单位： 淮海工学院

项目金额： 25万元

中文摘要： 量子点纳米被广泛应用于生物医学领域，其与铜离子都主要在肝脏蓄积，所以两者将共存于肝脏。前期研究已证实低/无毒水平的两种物质共存会产生协同作用，导致对肝细胞的高毒性。弄清两者协同作用的机理对肝脏毒性风险的屏蔽具有现实意义。 量子点容易结合药物分子实现对药物的胞内靶向运送，这种以量子点欺骗细胞膜识别系统为基础的技术被称为"木马技术"；量子点同样易于与铜离子结合并伴随荧光信号变化，也因此被用作铜离子的检测。 所以我们推断：量子点与铜离子共存环境中，量子点可能以同样的木马作用，结合并载带铜离子进入细胞，使细胞铜蓄积量增加，导致高的复合毒性，即是本研究假设的"木马机理"。 本研究以"木马机理"为中心，结合量子点的破膜和对铜解毒蛋白抑制作用，来解释低/无毒水平量子点与铜离子之间的协同作用机理，并对联合毒性的氧化应激路径进行探索以期对相关的复合毒性提供有效的屏蔽手段。

中文关键词： 量子点；Cu2+；木马角色；；

英文摘要： Quantum dots nanoparticles was recently developed as drug delivery tools due to its ability to transacross cell defensive system and, the high activity to bond drug molecular. The technique to use QDs as carrier taking drug into targeting organs is called trojan-horse technique resulted from the similar role of QDs. QDs also possess the ability to bond Cu2+, and these two chemicals can both primarily accumulate into liver, therefore we wondered if the similar trojan-horse role of QDs is responsible for the synergistic toxicity of QDs with Cu2+: QDs attach Cu2+, taking much more Cu2+ accumulate into cells and thus, the cell toxicity is enhanced. We aimed to test this hypothesis in this study. The presence of a low dose of mercaptosuccinic acid-coated cadmium telluride QDs (IC10 value of 2 μg/ml) in a Cu2+ solution (2.5-20 μg/ml: IC10~ IC40 value) caused a higher toxicity to human hepatic L02 cells，which has been proved in the previous study and is the basis of Trojan effects hypothesis. We aim to investigate the joint toxicity of QDs and Cu2+ to L02 cells based on the Trojan effects, which is also combined with the damaging effects of QDs to cell membrane and the inhibition of QDs to copper tranportor. Then the possible oxidative injury pathway of synergistic toxicity will also be investigated to provide the pot

英文关键词： QDs；Cu2+；Trojan role；；