桂枝、荆芥挥发油抗炎效应的NLRP3炎症小体通路机制研究

项目名称： 桂枝、荆芥挥发油抗炎效应的NLRP3炎症小体通路机制研究

项目编号： No.81473399

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 曾南

作者单位： 成都中医药大学

项目金额： 73万元

中文摘要： 前期研究证实，桂枝、荆芥挥发油抗炎、抗病毒效应的发挥与干预TLR2/4、TLR7信号转导通路有关。新近研究表明，NLRP3炎症小体作为连接炎症与免疫的重要纽带，该复合体的活化是IL-1β等促炎因子成熟的关键环节，被认为是感染性、炎症性疾病的治疗新靶点，且与胞膜TLR的激活存在密切联系。本研究在中医药理论指导下，基于病原微生物感染、炎症反应与表证密切相关，挥发油是解表药辛味的主要物质基础的基本事实，围绕桂枝、荆芥挥发油的抗炎目标效应，选择NLRP3炎症小体为切入点，以炎性细胞/组织-炎性细胞因子-信号转导为主线，采用体外与体内实验相结合研究模式，运用荧光定量PCR、Western blot、基因芯片、细胞荧光成像分析等方法技术，以NLRP3炎症小体激活通路途径的若干关键靶分子、产物为评价指标，进一步探索桂枝、荆芥挥发油的抗炎效应的分子机制，有利于丰富解表药辛散解表性效的科学内涵。

中文关键词： 荆芥；桂枝；挥发油；抗炎；NLRP3炎症小体

英文摘要： Early studies have confirmed that the mechanisms of anti-inflammatory and antiviral effects of volatile oil of Guizhi and Jinjie were related to interfereing the signal transduction pathway of TLR2/4 and TLR7. Recent studies have shown that NLRP3 inflammasome is an important link between inflammation and immune response，that activation of NLRP3 inflammasome is key link of IL-1β maturation, and be considered as the new treatment target on infectious and inflammatory diseases. It also has close connection with TLR activation. Under the guidance of TCM theory, and based on the pathogenic microorganism infection, inflammation is closely related to the Exterior syndrome , and volatile oil are the main material basis of drugs for relieving Exterior Disorder that show pungent flavour characteristic，this study will further explain the mechanism of anti-inflammatory action of volatile oil of Guizhi and Jinjie from NLRP3 inflammasome pathway. In study, we will choose NLRP3 inflammasome as the pointcut, and make inflammatory cell/tissue - inflammatory cytokines - signal transduction as the main line, use the study mode of combining in vitro with in vivo experiments, to evaluate the expressions of the key target molecules or products in NLRP3 inflammasome activation pathways by using the methods of fluorescence quantitative PCR, Western blot, gene chip and cell fluorescence image analysis. The results will further explore the molecular mechanism of anti-inflammatory action of volatile oil of Guizhi and Jinjie, meanwhile rich the scientific connotation of xinsan jiebiao efficacy of drugs for relieving Exterior Disorder. …

英文关键词： Jinjie(schizonepetae);Guizhi(ramulus cinnamomi);volatile oil;anti-inflammatory;NLRP3 inflammasome