项目名称: 油菜素甾醇调控细胞骨架的细胞和分子机制研究
项目编号: No.31271300
项目类型: 面上项目
立项/批准年度: 2013
项目学科: 生物科学
项目作者: 王学路
作者单位: 华中农业大学
项目金额: 95万元
中文摘要: 植物激素油菜素甾醇(BRs)和细胞骨架在植物细胞的发育过程中都起着重要作用。BR的合成及信号通路组分的突变体往往表现出细胞骨架结构被破坏的表型,并因此引起细胞的形态和植物发育的异常。但是对于植物中,BR信号如何调控植物细胞的形态和生长,尤其是BR信号调控细胞骨架的机制所知甚少。在前期研究中,我们采用液相层析和串联质谱的方法,通过分离和鉴定与BR信号通路重要组分BIN2互作的蛋白,发现BIN2与多种微管蛋白和微管结合蛋白相互作用,因此我们推测,BR信号可能通过BIN2直接调控这些微管和微管结合蛋白,来调控细胞的形态和大小。在本研究中,我们将采用细胞生物学、遗传学和生物化学的方法,验证BIN2与多种细胞骨架相关蛋白的互作关系,系统研究BR信号调控细胞形态和生长的详细机制,为深入研究植物激素调节植物生长发育的机制提供重要的理论指导。
中文关键词: 油菜素甾醇;细胞骨架;蛋白激酶;信号转导;植物发育
英文摘要: Brassinosteroids (BRs), a group of plant steroid hormones, and cytoskeleton both play important roles in regulating plant development.Deficiency in BR biosynthesis or signaling leads to abnormal plant phenotypes similar to that of microtubule and microfilament defective mutants. However, we know little about how BRs regulate abnormal cytoskeleton and cell morphological defect. Recently, we have identified many tubulins and their associated proteins interacting with BIN2 by LC-MS/MS. Therefore, we hypothesize that BRs may regulate plant cell shape and size through regulating tubulins and tubulin associated proteins. In this research, we will apply cell biology, genetic and biochemical appraoches to explore the relationship between BIN2 and cytoskeleton proteins to disclosure the mechanism by which how BR regulate plant cell shape and growth. Our study will provide significant insight into how phytohormones regulate plant development.
英文关键词: brassinosteroids;cytoskeleton;protein kinase;signal transduction;plant development