牛磺酸抑制AS肉鸡右心肥大过程中calpains介导细胞凋亡作用的研究

项目名称： 牛磺酸抑制AS肉鸡右心肥大过程中calpains介导细胞凋亡作用的研究

项目编号： No.31502026

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 杨群辉

作者单位： 沈阳农业大学

项目金额： 20万元

中文摘要： 肉鸡腹水综合征(Ascites Syndrome，AS)是导致肉鸡死亡的主要原因之一，可造成严重的经济损失，抑制右心肥大是防治AS的有效手段。项目组前期研究发现，牛磺酸可通过降低肉鸡心肌μ-calpain表达和细胞凋亡，抑制右心肥大并降低AS发病率，提示其作用与抑制calpains介导的细胞凋亡密切相关，但确切机制尚不明确。本项目拟通过低温诱导肉鸡腹水症模型，结合体外心肌细胞培养，采用免疫组化技术和TUNEL法检测右心肥大和心肌细胞凋亡情况，明确牛磺酸对AS右心肥大和心肌细胞凋亡的抑制作用；采用流式细胞术、荧光分光光度法、QPCR、Western-blot等技术检测心肌细胞 [Ca2+]i、calpains活性、calpains介导的凋亡通路中相关因子表达量的变化，阐明牛磺酸对AS右心肥大过程中calpains介导细胞凋亡的抑制作用及其机理，为应用牛磺酸防治AS提供科学的理论依据。

中文关键词： 牛磺酸；右心肥大；细胞凋亡；钙蛋白酶；肉鸡

英文摘要： Ascites Syndrome (AS) is the main reason for the death of broilers, and it could cause serious economic loss to the poultry industry. Inhibition of the right ventricular hypertrophy is an effective measures to prevent and cure AS. Our previous studies proved that taurine could prevent myocardial hypertrophy by decreasing the expression of μ-calpain and cell apoptosis during the process of ascites syndrome (AS) in broilers, which indicates the effect of taurine on AS has close relationship with calpains mediated cell apoptosis, however the mechanisms of which is still unclear. The present study aims to explore the inhibition effects of taurine on right ventricular hypertrophy and myocardial cell apoptosis through inducing broiler AS by low temperature, durning which the relevant index of right ventricular hypertrophy and cell apoptosis were monitored. In order to research the inhibition mechanism of taurine on the calpains mediated cell apoptosis, the free intracellular ca2+ concentration and the activity of calpains were detected by flow cytometry and fluorescence spectrophotometry, the gene and protein expressions of calpains and the downstream of calpains mediated cell apoptosis pathways were detected by real-time quantitative PCR and Western blot. And then through cell culture in vitro, the prevention effect of taurine on myocardial hypertrophy was further verified by detecting the expression changes of gene and protein on the downstream of calpains mediated cell apoptosis pathways. Finally, the role of taurine on calpains mediated cell apoptosis pathways in the process of right ventricular hypertrophy and the inhibition mechanisms of taurine on the pathways would be elucidated. The results will provide a reference for the application of taurine on the prevention and treatment of AS.

英文关键词： taurine;right ventricular hypertrophy ;apoptosis;calpains;broilers