基于磁共振动态增强灌注成像的肝癌血管生成拟态图像纹理特征提取与定量

项目名称： 基于磁共振动态增强灌注成像的肝癌血管生成拟态图像纹理特征提取与定量

项目编号： No.81501433

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 李若坤

作者单位： 复旦大学

项目金额： 18万元

中文摘要： 血管生成拟态(VM)指肿瘤细胞通过自身塑形而形成的管道样结构,是独立于内皮依赖性血管的微循环模式。VM形态学及血流动力学与内皮依赖性血管存在明显差异，不适于以内皮细胞为靶标的抗血管生成治疗。本研究拟建立裸鼠MHCC97-H肝癌移植瘤模型,在不同时间点(种瘤1、2、3、4周)行动态增强灌注成像（DCE-PWI）,获得Ktrans、 Ve和 Kep等灌注值；采用Mazda图像纹理分析软件，利用灰度共生矩阵（GLCM）纹理分析技术提取T1mapping灌注图像纹理特征，获得ASM、Con、Cor、ENT、IDM和V等纹理参数；并与病理VM数目行相关性分析,比较VM阳性组与阴性组各灌注值及纹理参数差异。本研究拟利用先进的纹理分析技术最大化挖掘DCE-PWI所反映的肝癌微循环信息，寻找能够在体无创性定量评价肝癌VM生成的影像生物标记物，为肝癌个体化抗血管生成治疗方法的建立提供实验依据。

中文关键词： 肝细胞肝癌；血管生成；纹理分析；灌注加权成像；磁共振成像

英文摘要： Vasculogenic mimicry (VM) is defined as the pattern of vessels lined exclusively with tumor cells mimicking the presence and function of endothelial cells.The morphology and hemodynamics of VM is different from that of endothelial-dependent vessel. VM is associated with high tumor grade, invasion and metastasis, poor antiangiogenesis response, and short survival in patients with hepatocellular carcinoma (HCC). In the present study, HCC xenograft nude mouse models with potential of VM formation will be established and undergo dynamic contrast-enhanced perfusion weighted MR imaging (DCE-PWI) using a 3.0T MR scanner. Quantitative perfusion parameters were measured on T1mapping including Ktrans, Ve and Kep values. Texture features will be extracted from T1mapping perfusion images using Mazda image texture analysis software including ASM、Con、Cor、ENT、IDM and V values. VM will be quantified using CD34/PAS immunohistochemical staining at pathology. The correlation analysis will be performed between perfusion parameters, texture features and VM number. The perfusion parameters and texture features of VM positive group were compared with those of VM negative group. Our study intend to utilize advanced texture analysis method to maximize the microcirculation information on DCE-PWI, identify valuable imaging biomarkers for quantification of VM, and provide experimental evidence for personalized treatment in HCC patients.

英文关键词： hepatocellular carcinoma;angiogenesis;texture analysis;perfusion weighted imaging;magnetic resonance imaging