纳米银广谱抗病毒作用及机制研究

项目名称： 纳米银广谱抗病毒作用及机制研究

项目编号： No.30870681

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 金属学与金属工艺

项目作者： 郑丛龙

作者单位： 大连大学

项目金额： 30万元

中文摘要： 本项目利用细胞生物学和分子生物学等实验方法，探讨了纳米银对RNA病毒、DNA病毒和反转录病毒的广谱抑制作用，并从病毒的蛋白酶和病毒核酸的结构及功能等分子水平，探讨了纳米银广谱抗病毒的作用机制。 研究结果证实，纳米银对多种呼吸道病毒（流感病毒、副流感病毒、腺病毒、呼吸道合胞病毒）、疱疹病毒和HIV有明显的抑制作用。这种抑制作用具有非选择性和广谱性。动物实验证实纳米银对流感病毒感染有良好的治疗作用和预防作用。动物实验还证实纳米银有良好的生物安全性。研究结果提示，纳米银广谱抗病毒的作用机制与纳米银作用于病毒表面的蛋白、蛋白酶活性和病毒核酸有关。此外，纳米银对多种病毒体颗粒有明显的破坏作用。 本项目研究期间，发表了9篇研究性论文，1其中1篇为SCI收录论文；申报了2项发明专利；本项目研究期间与澳大利亚迪肯大学建立了良好的科研合作关系。 本项目的研究证实了纳米银广谱抗病毒的作用并初步揭示了纳米银广谱抗病毒的作用机制，这些研究结果为研制和开发安全有效、黏膜局部使用的广谱抗病毒纳米银药物和材料提供理论基础和实验依据，为黏膜局部预防和治疗人类呼吸道和泌尿生殖道病毒感染提供新的方法和启示。

中文关键词： 纳米银；呼吸道病毒；广谱抗病毒；作用机制

英文摘要： This project using the experiment methods of cell biology and molecular biology discussed the inhibition effects of broad spectrum of silver nanoparticles on the RNA virus , DNA viruses and retrovirus and from the molecular level of virus nucleic acid and protease discussed the broad-spectrum antiviral mechanism of silver nanoparticles. The results showed that the silver nanoparticles have apparent inhibition effects to a variety of respiratory virus (influenza virus, parainfluenza virus, adenovirus, respiratory syncytial virus), herpes virus and HIV and the inhibition effects are the nonselective and broad spectrum. Animal experiments confirmed that silver nanoparticles have good treatment effect and prevention role in influenza virus infection Animal experiments also confirmed that silver nanoparticles a good biological safety. The results suggest that nano silver's broad-spectrum antiviral mechanisms are associated with the actions of silver nanoparticles in the virus protein, the viral nucleic acid and viral protease activity. In addition, the nanosilver has obvious damage function to a variety of virus particles. The project during the study, published 9 research papers , including 1 article for SCI included paper; Declared the 2 invention patents; This research project established a good relationship of scientific research with Australia Deakin university. This project research confirmed that the role of the silver nanoparticles broad-spectrum antiviral and initially revealed the nanosilver broad-spectrum antiviral mechanism of action, and these findings provided theoretical foundation and experimental basis for the research and development of the local-use broad-spectrum antiviral drugs and silver nanoparticles materials. These findings also provided new ways and enlightenments for the prevention and treatment of viral infections in human respiratory and the genitourinary tracts.

英文关键词： Nano Silver; respiratory viruses; broad spectrum; action mechanism