一群以B7-H3+CD14+为特征的肿瘤相关巨噬细胞亚群在肾癌进展中的作用机制研究

项目名称： 一群以B7-H3+CD14+为特征的肿瘤相关巨噬细胞亚群在肾癌进展中的作用机制研究

项目编号： No.81472401

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 侯建全

作者单位： 苏州大学

项目金额： 72万元

中文摘要： 肿瘤微环境中大量的间质细胞，特别是肿瘤相关巨噬细胞（TAM）在肿瘤进展中发挥着重要作用。鉴于髓源性细胞的异质性，不同亚群发挥的功能并不相同，因此鉴定不同的TAM亚群对研究该细胞群在肿瘤进展中的作用尤为重要。本项目前期工作中发现了一群以B7-H3+CD14+为特征的新型TAM亚群，该亚群主要在肾癌灶聚集，呈现肿瘤组织特异性，因此推测该亚群可能是真正意义上的肿瘤相关巨噬细胞。尽管形态学和基因表达方面存在显著差异，但B7-H3-和B7-H3+CD14+TAM亚群功能特征并不清楚。本项目拟通过临床病例分析和体内外实验，从T细胞免疫应答、肿瘤侵袭转移和肿瘤新生血管形成等三个方面入手，比较分析B7-H3+和B7-H3-CD14+亚群表达的临床意义意义、生物功能及其在肾癌进展中的作用机制。

中文关键词： C12_肾；肾盂；输尿管肿瘤；肿瘤相关巨噬细胞；B7-H3

英文摘要： Tumor-associated macrophage (TAM)， among stroma cells in tumor microenvironment, plays an important role in development of tumor. Based on specialty of myeloid-derived cells, different subtypes of cells showed different functions. Thus, it is particularly important to characterize various subtypes of TAM in study of cellular roles in the tumor development and cancer progress. Our preliminary studies found a novel group of TAM subtypes with B7-H3+CD14+. This subtype of cells was located mainly in the tissue of renal cancer. Those cells showed their specialties to cancer tissues. Therefore, we hypothesize that the type of cells could be critical tumor-associated macrophage. There are significant differences in morphology and expression of the genes between TAM subtype B7-H3+ and B7-H3-CD14+. However, their cellular functions are largely unknown. This proposed project will use analysis of clinical cases and in vivo as well as in vitro experiments focus on immuno-responses of T cells, tumor-invasion and metastasis, and tumor angiogenesis. Clinical significance for expression of B7-H3+ and B7-H3-CD14+ subtypes will be analyzed and compared. Their bio-functions and mechanisms in the development of renal cancer will be investigated. Information gained will add new knowledge in the fields.

英文关键词： Renal cancer;tumor-associated macrophage;B7-H3