脑心通调控ADP信号途径(P2Y12通路)抗血小板作用的机制

项目名称： 脑心通调控ADP信号途径(P2Y12通路)抗血小板作用的机制

项目编号： No.81202798

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 华声瑜

作者单位： 天津中医药大学

项目金额： 23万元

中文摘要： 血栓性疾病严重威胁着人类健康,内皮损伤后,早期血小板黏附/活化引起的级联反应是血栓形成的始动环节和核心环节。课题组在前期研究工作基础上（NO30801499；200800630001），以"早期血小板黏附/活化"作为血栓形成的始动环节入手,以ADP信号途径-P2Y12通路为切入点,选用临床治疗血栓性疾病行之有效的经典复方"步长脑心通"的水溶性组分和脂溶性组分，通过血小板体外实验，采用比浊法，酶联免疫吸附法，流式细胞术等现代医学研究方法，确定脑心通有效组分对血小板活化的作用通路并验证具体作用靶点；通过在体实验,采用TTC染色检测梗死面积,测定出血时间,进一步验证其组分对MCAO模型大鼠治疗的有效性和安全性,最终阐明脑心通水溶性组分和脂溶性组分抗血小板作用的分子机制和作用靶点；比较脑心通水溶性组分和脂溶性组分对血小板活化的抑制作用,对中药二次开发提供理论和实验依据,意义重大并具较好社会效益。

中文关键词： 脑心通；血小板；二磷酸腺苷；信号通路；机制

英文摘要： People are suffering from thrombolic disease , after endothelial injury, early platelet adhesion / activation induced by cascade is thrombosis of the actuating link and key link. The research group on the previous research work based on the ( NO30801499; 200800630001)," early platelet adhesion and activation" as a thrombosis actuating link proceed with, to the signal pathway of ADP signaling pathway(P2Y12) as the breakthrough point, we use the effective classic compound" Buchang Naoxintong" water-soluble component and a lipophilic component, through platelet experiments in vitro, using Borns, enzyme-linked immunosorbent assay, flow cytometry and other modern medical research methods, to determine the effect of Naoxintong effective components on platelet activation pathway and verify the specific target; through the experiment in vivo, TTC staining was used to detect infarct size, determination of bleeding time, further validation of its components on the MCAO in a rat model of effectiveness and safety of treatment, eventually shed Naoxintong water-soluble fractions and lipophilic fractions of the antiplatelet effect of molecular mechanisms and targets; comparison of Naoxintong water-soluble fractions and lipophilic fractions on antiplatelet activation,to provide the theoretical and experimental basis of the twic

英文关键词： Naoxintong；platelet；ADP；signaling pathway;；mechanism