牛磺酸对慢性应激大鼠海马神经再生的作用及机理研究

项目名称： 牛磺酸对慢性应激大鼠海马神经再生的作用及机理研究

项目编号： No.31302051

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 吴高峰

作者单位： 沈阳农业大学

项目金额： 25万元

中文摘要： 现代快节奏的工作生活压力等慢性应激导致的海马神经再生障碍已被证明是引起记忆减退、抑郁等精神疾病的主要原因之一。牛磺酸是幼龄动物神经发育的必需物质，并可促进多种干细胞增殖。课题组研究发现，牛磺酸可改善成年大鼠海马神经元结构和功能；并初步证明可抑制脂多糖导致的大鼠海马神经再生障碍，促进神经干细胞增殖，但其对慢性应激海马神经再生的确切作用及机制尚不明确。本项目拟制备慢性应激大鼠模型结合海马神经干细胞培养，观测大鼠学习记忆及焦虑行为，应用免疫组化技术检测神经再生各阶段标记物的表达，评价牛磺酸对慢性应激导致神经再生障碍的保护作用；采用高效液相电化学和ELISA法检测神经递质及激素水平，Real-Time PCR、Western-blot技术检测海马神经生长和营养因子及相关信号通路各因子的基因、蛋白表达水平，阐明牛磺酸对慢性应激大鼠海马神经再生的调控机制，为神经再生障碍相关疾病的防治提供参考。

中文关键词： 牛磺酸；慢性应激；海马；神经凋亡；神经再生

英文摘要： Chronic psychological and physiological stess which was induced by the quick pace of mordern life could induce mental disease such as hypomnesia and depression by damaging hippocampal neurogenesis.Taurine was known to be necessary in the neurodevelopment of young animals and could promote the proliferation of various kinds of stem cells from young animals. Our previous research found that taurine could improve learning ability and memory of adult rats by improving the structure of neuron and synapses and regulating neurotransmitters.Further research proved that taurine could counteract the suppressive effects of lipopolysaccharide on neurogenesis in the hippocampus of rats by increasing the proliferation of neuronal precursor cells in the Dentate Gyrus of hippocampus. Whereas the effects and mechanisms of taurine on chronic stress animals is still unknown. The present research was conducted to clarify the effects and its mechanisms of taurine on neurogenesis in the hippocampus by examining the learning ability, memory and anxiety behavior of rats under chronic stress conditons. Neurotransmitters and hormons participated in the process of neurogenesis in blood and hippocampus were analyzed by way of High Performance Liquid Chromatography and ELISA. Expression levels of protein markers in different stages of neur

英文关键词： Taurine；Chronic stress；Hippocampus；Neuronal apoptosis；Neurogenesis