香蕉MaOFP介导的MuMADS1对果实成熟及品质形成的调控机制

项目名称： 香蕉MaOFP介导的MuMADS1对果实成熟及品质形成的调控机制

项目编号： No.31471924

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 刘菊华

作者单位： 中国热带农业科学院热带生物技术研究所

项目金额： 85万元

中文摘要： 香蕉是世界重要热带水果，果实采后成熟及品质形成调控是决定香蕉产业可持续健康发展的关键科技问题，但目前其形成调控的机制不明。MADS-box在果实成熟及品质形成中具有重要作用，研究MADS-box转录因子与其它转录因子互作的分子机制是解决这一问题的有效途径之一。我们在前基金资助下获得的证据表明：香蕉MuMADS1与MaOFP相互作用。本项目采用分子生物学和分子遗传学手段研究MuMADS1与MaOFP互作的分子机制，研究MaOFP介导的MuMADS1对果实品质的调控作用，研究在突变体背景下MuMADS1与MaOFP过表达果实中转录组的变化，分析MaOFP介导的MuMADS1所转录调控的基因的功能，阐明果实品质形成过程中转录调控因子的作用机制。研究结果将为进一步采用分子生物学和基因工程手段通过调控这些位点而达到改善香蕉果实品质提供理论依据，也为其它以果实为收获对象的园艺植物品质改良提供借鉴作用。

中文关键词： 香蕉；MaOFP；MuMADS1；果实品质；调控机制

英文摘要： Banana is one of the most important tropical fruits in the world.Regulation of postharvest banana fruit ripening and quality formation is the key science and technology problem which decides the sustainable and healthy development of banana industry. However, the regulatory mechanism of fruit ripening and quality formation remains unclear. MADS-box transcriptional factors played important roles in fruit ripening and quality formation. Investigating the molecular mechanism of MADS-box transcriptional factor and other transcriptional factors' interaction is an effective way to solve this problem. Evidence we obtained under the previous National Natural Science Fundation of China (NNSFC) project supported showed that banana MuMADS1 interacted with an Ovate Family Protein named MaOFP(Musa acuminata Ovate Family Protein). This project is going to further investigate the molecular mechanism of MuMADS1 and MaOFP interaction , confirm the regulatory role of MaOFP-mediated MuMADS1 on fruit quality formation and investigate the transcriptome changes in over-expressed MuMADS1 and MaOFP fruits under mutant background by molecular biology and molecular genetics methods. The functions of genes transcriptionally regulated by MaOFP-mediated MuMADS1 were analysed. The purpose of this project is to understand the regulatory mechanism of transcriptional factors for fruit quality formation. These findings will provide theoretical basis for improving banana fruit quality through regulating the expressions of MuMADS1 and MaOFP by molecular biology and genetic engineering methods and also provide reference for genetic improvement of other horticultural plants whose harvest objects are fruits.

英文关键词： banana;MaOFP;MuMADS1;fruit quality;regulatory mechanism