可用于骨组织修复的多功能可注射型聚氨基酸基水凝胶的研究

项目名称： 可用于骨组织修复的多功能可注射型聚氨基酸基水凝胶的研究

项目编号： No.51203079

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 有机高分子材料学科

项目作者： 伍国琳

作者单位： 南开大学

项目金额： 25万元

中文摘要： 针对目前骨组织再生临床手术存在的不易塑性，易诱发炎症和修复效果差等问题，本项目拟设计合成一种具有良好生物相容性和生物可降解性的双组份可注射水凝胶，用于骨组织修复。选用分别具有酰肼基和醛基侧链的聚氨基酸作为注射前体，通过温和条件下，原位发生腙键交联，形成水凝胶。体系可同时负载成骨细胞、生长因子和药物等，加速骨组织重建和完善术后效果。聚氨基酸衍生物采用聚琥珀酰亚胺开环的方法合成，通过选择不同的开环试剂将功能基团引入侧链。通过调节侧基的种类、数量和混合条件、交联度等调控凝胶的孔结构、机械性能和降解性能，模拟创伤部位组织情况，诱导骨生长。利用腙键接枝环糊精调节凝胶的孔径和孔隙率，并实现同时负载和控制释放多种亲、疏水药物的目的。探索水凝胶中负载药物的释放性能和细胞的增殖情况，评价材料的生物相容性和骨组织修复治疗效果。通过本项目研究，有望开发可用于骨组织修复的新型多功能可注射水凝胶产品。

中文关键词： 聚氨基酸；水凝胶；组织工程；生物降解；可注射

英文摘要： In order to deal with the problems in bone tissue regeneration, such as difficult to plastic, easy to induce inflammation and immune response, an injectable poly(amino acid) based hydrogel via hydrazone crosslinking strategy is designed to connect or fill the broken tissues. The gelation occurs under physiological conditions immediately upon mixing of the two aqueous poly(amino acid) derivatives with aldehyde and hydrazide functional groups without aids of crosslinking agents or catalysts. Poly(amino acid)s have excellent biocompatibility, biodegradability and various side groups for further functionalization, which have wide ranged of potential biomedical applications. Unlike most other polypeptides prepared by complicated ring-opening polymerization of amino acid N-carboxyanhydrides(NCAs), Poly(α,β-aspartic acid)(PAsp) derivatives can be easily synthesized by ring-opening reactions of polysuccinimide(PSI), which can be synthesized by thermal polycondensation of L-aspartic acid. In this project, the two injected components have the same polypeptide backbone, which provides a constantly biodegradation. This injectable hydrogel can be loaded with antibiotics, growth factor or osteoblasts to improve treatment. The gelation, morphology, biocompatibility, degradation pro?les and agent release behaviors of PAsp-base

英文关键词： poly(amino acid)s；hydrogel；tissue engineering；biodegradation；injectable