刺参对蛋白质营养干预的应答机制

项目名称： 刺参对蛋白质营养干预的应答机制

项目编号： No.41506192

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 天文学、地球科学

项目作者： 夏苏东

作者单位： 天津市水产研究所

项目金额： 21万元

中文摘要： 刺参养殖业发展迅速，单种产值列海水之冠，但相关营养与饲料学基础和应用基础研究相对薄弱，蛋白质是水产动物最重要营养元素之一，刺参健康养殖、生态型营养饲料研究与开发，亟需蛋白质营养对刺参代谢影响机制的基础研究。本项目在前期研究基础上，利用传统营养学方法，建立刺参不同饲料蛋白质水平下氮收支方程，探寻饲料氨基酸与组织氨基酸的关系。立足代谢组学研究方法，利用代谢指纹谱分析技术，测定不同蛋白质营养条件下刺参组织中代谢指纹图谱，筛选差异代谢物，揭示蛋白质营养相关的代谢通路，推测代谢途径变化；利用靶标分析技术，选择重要差异代谢物进行定量分析，研究刺参关键代谢通路的精确变化，总结刺参对蛋白质营养干预的应答，从营养与饲料学和代谢组学角度全面阐述蛋白质营养对刺参影响的生物学机制。该成果将为刺参蛋白质营养需要量精确确定、生态型营养与饲料的研究与开发提供重要的理论支持，为水产动物营养生理学研究提供新思路、新方法。

中文关键词： 刺参；蛋白质；代谢组学；代谢指纹谱分析；靶标分析

英文摘要： The culture of sea cucumber (Apostichopus japonicas) skyrocket increase rapidly, and the production value reached the top of the whole single species in the marine aquaculture. However, the nutrition and basic application research was relatively week. Protein was one of the most important nutrition factors in feed. The research and development of compound feed was in urgent need of the basic research of dietary protein nutrition metabolic mechanism in sea cucumber. Based on our previous research, in this study we will evaluate the effect of dietary protein level on food nitrogen, feces nitrogen, excretion nitrogen and growth nitrogen, the composition of amino acid in feed and body, and then build the nitrogen budget, using traditional nutrition and feed science technology. Based on metabolomics, the small molecule metabolites in body fluid and body wall of different dietary protein level groups would be measured to reveal the influence of dietary protein level on the endogenous metabolites. The dietary protein nutrition related potential biomarkers will be screened, and the change of nitrogen metabolic pathway will be speculated. In all, the effect of dietary protein levels on mechanism of nitrogen metabolism in sea cucumber will be studied from nutrition and feed science, and metabolomics perspectives. The results would provide important theoretical support for the determination of protein requirement and development of eco-nutrion compound feed. They also would provide new ideas and new methods for the research of aquatic animal nutrition and feed.

英文关键词： Apostichopus japonicas;Protein ;Metabolomics;Metabolic Fingerprinting Analysis;Target Analysis