前列腺癌PIA-PIN-PCa恶性转化中标志性lncRNA功能及其调控网络研究

项目名称： 前列腺癌PIA-PIN-PCa恶性转化中标志性lncRNA功能及其调控网络研究

项目编号： No.81460389

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 王共先

作者单位： 南昌大学

项目金额： 47万元

中文摘要： 前列腺癌(PCa)是影响老年男性生活质量和预期寿命的重要疾病。目前有学者提出增生性炎性萎缩(PIA)-前列腺上皮内瘤(PIN)-PCa恶性转化可能是PCa发生的新模式。申请者前期研究也证实PIA与PCa密切相关。长链非编码RNA(lncRNA)是目前研究热点及新兴领域，其参与了PCa发生、发展及转移，同时也是PCa分子标志物。申请者推测lncRNA可能也参与了PIA-PIN-PCa恶性转化进程。本研究拟采用新一代lncRNA芯片检测正常前列腺腺上皮、PIA、PIN和PCa组织中差异性表达lncRNA，分析其调控网络，并通过随访及检测其表达，以寻找新的标志性lncRNA；knockdown及过表达标志性lncRNA后，体内外实验观察PCa细胞的增殖、侵袭与转移能力的变化，明确其功能，为早期干预PCa疾病进程提供新的治疗靶点；并为PIA-PIN-PCa恶性转化模式提供新的科学证据。

中文关键词： C14_前列腺肿瘤；增生性炎性萎缩；前列腺上皮内瘤；长链非编码RNA

英文摘要： Prostate cancer(PCa) is an important disease affecting elderly men of life quality and life expectancy. Some scholars have proposed the malignant transformation of Proliferative inflammatory atrophy(PIA)-Prostatic intraepithelial neoplasia(PIN)-PCa might be a novel model in the development of PCa, while applicants also confirmed the significant correlation between PIA and PCa in previous studies. Recent researches have been focusing on the merging field of long non-coding RNA(lncRNA) which involves in the onset, development and metastasis of PCa, and also acting as biomarker for PCa. Applicants suppose that lncRNA may get involved in the malignant transformation of PIA-PIN-PCa. Our study aims to detect differential expression of lncRNA between normal prostate glandular epithelia, PIA, PIN and PCa by the latest lncRNA microarry; analyse corresponding regulatory networks; seek for novel hallmark lncRNA by following up and detecting its expression. After the hallmark lncRNAs are knockdowned or overexpressed, its functions should be identified by observing the changes of proliferation, invasion and metastatic ability of PCa cells in vivo and in vitro so as to provide therapeutic targets for early intervening PCa progress as well as offer novel scientific evidence for malignant transformation of PIA-PIN-PCa model.

英文关键词： Prostate cance;Proliferative inflammatory atrophy;Prostatic intraepithelial neoplasia;Long non-coding RNA