从稳定细胞外基质探讨健脾补土法抗脑缺血损伤机制

项目名称： 从稳定细胞外基质探讨健脾补土法抗脑缺血损伤机制

项目编号： No.81202632

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 李花

作者单位： 湖南中医药大学

项目金额： 23万元

中文摘要： 神经细胞外基质（ECM）对神经细胞有支撑、营养与保护作用，与中医脾土"主承载"，滋生万物的功能相似，健脾补土法保护ECM为抗脑损伤开辟了新方向。本项目在前期健脾补土法改善临床症状，减少神经元丢失，增加层粘连蛋白表达和减少基质金属蛋白酶（MMPs）-9的表达的基础上，采用脑缺血大鼠模型和嗅鞘细胞缺氧损伤模型，以ECM为切入点研究健脾补土法组方对脑组织病理形态、血脑屏障、脑组织ECM的保护作用以及对MMPs及其抑制剂(TIMPs)、纤溶酶原激活物(PA)及其抑制剂(PAI)表达的影响，进一步从影响ECM降解的NF-κB通路探讨健脾补土法保护ECM的机理。本研究将ECM与中医脾土的若干相似点进行对照，运用取类比象思维，提出健脾补土法治法，重视培补后天脾土，为脑缺血性疾病提供了新的治法；重视保护ECM，为脑卒中治疗提供了新的思路和靶点，对进一步开发新的中医药治疗方法，提高疗效具有潜在的应用价值。

中文关键词： 脑缺血；细胞外基质；健脾补土；基质金属蛋白酶；NF-κB通路

英文摘要： Extra cellular matrix（ECM）is the living environment of neuron, it provide supporting, nutrition, protecting for neuron, it have some similarity as the carry and breeding of spleen in traditional Chinese medicine. Protecting ECM by the method of Invigorating the Spleen opens new avenues for protecting neurocyte, reducing damages of cerebral ischemia.Using cerebral ischemia-reperfusion rats models and hypoxic injured olfactory ensheathing cells models, taking ECM as a key, this project will base on our previous working basis that the method of Invigorating the Spleen can improve the clinical symptoms, reduce Neuron loss, increase the express of LN and decrease the express of MMPs-9 to systematically study the protective effects on cerebral histopathology, blood brain barrier, ECM in Brain Tissue and on the expression of matrix metalloproteinase and it's inhibitor(tissue inhibitor of meta11oproteinase-1,TIMPs), plasminogen activator(PA)and it's inhibitor(PAI) of Invigorating the Spleen Therapy composition. Among the cellular signal pathways, NF-κB pathway is the decisive pathway for ECM degradation. So we will investigate the relationship between the effects on protecting ECM and regulateing NF-κB pathway of Invigorating the Spleen Therapy furthermore.Contrasting the similar betweem ECM and spleen in traditional Ch

英文关键词： Cerebral ischemia；Extracellular matrix；Invigorating the spleen therapy；Matrix metalloproteinases；NF-κB passway