猴头菇多糖对氧化应激状态下猪小肠上皮通透性的调节及其机制

项目名称： 猴头菇多糖对氧化应激状态下猪小肠上皮通透性的调节及其机制

项目编号： No.31502110

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 水产学、兽医学

项目作者： 李健

作者单位： 福建农林大学

项目金额： 20万元

中文摘要： 集约化养殖模式造成生猪生产过程中肠道氧化应激日益严重，在氧化应激状态下，小肠上皮通透性随之改变，导致吸收功能和屏障作用受损，从而引起猪群健康度明显降低，直接影响生产性能。本课题组前期研究工作表明，猴头菇多糖能够显著改善呼肠孤病毒感染导致的番鸭肠道抗氧化能力降低和上皮结构破坏等病理变化。借鉴前期研究工作的基础，针对生猪肠道氧化应激和通透性变化这一问题，本项目拟分别建立猪小肠上皮细胞（IPEC-J2）和仔猪的氧化应激模型，研究猴头菇多糖对于氧化应激造成的猪肠道上皮通透性异常变化的干预作用，分析在氧化应激状态下由细胞凋亡介导的蛋白酶激动受体-1（PAR-1）对于细胞通透性的调节机制；初步明确氧化应激与猪小肠上皮通透性的关系，阐明猴头菇多糖对这一病理性变化的调节作用和机制。研究结果不但为生猪肠道保健提供新思路，也可以为促进猴头菇多糖在防治生猪肠道氧化应激方面的应用提供科学依据。

中文关键词： 多糖；抗氧化；抗应激；信号通路

英文摘要： Intensive swine cultivation results in severe intestinal oxidative stress, which led to the change of the intestinal epithelial permeability, the destruction of the intestinal absorption and defense, and significantly reduction for health degree and productive performance. Our previous work has shown that Hericium polysaccharide (HEPS) can significantly improve the intestinal epithelial antioxidant capacity and reduce their structural damage of the muscovy duck infected by reovirus. Aiming at the increasingly serious intestinal problem of permeability and oxidative stress, based on the preliminary studies, the oxidative stress models of the porcine intestinal epithelial cells (IPEC-J2 cell) and piglet will be established, to study the intervention effect of HEPS against the abnormal changes of the swine intestinal epithelial permeability caused by oxidative stress, and to analyze the permeability regulatory mechanism of protease-activated receptor 1 (PAR-1) mediated by apoptosis. The purpose of this study is to preliminarily define the relationship between oxidative stress and porcine intestinal epithelial permeability, and to clarify the regulation and mechanism of HEPS against this pathological changes. The results can provide new ideas for the pig intestinal healthcare and promote the usage of HEPS for the prevention and treatment of porcine intestinal oxidative stress.

英文关键词： Polysaccharide;Antioxidant;Anti-stress;Signaling pathway