基于微流控技术的多肿瘤标志物电化学免疫传感器

项目名称： 基于微流控技术的多肿瘤标志物电化学免疫传感器

项目编号： No.61471342

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 无线电电子学、电信技术

项目作者： 罗金平

作者单位： 中国科学院电子学研究所

项目金额： 83万元

中文摘要： 多肿瘤标志物联合快速检测对肺癌的早期诊断具有重要的研究意义和临床需求；目前临床主要采用中心实验室的大型分析仪器和试剂盒进行检测分析，需要专业人员操作、检测周期较长。本项目选择CEA、CYFRA21-1、NSE和ProGRP作为研究切入点，将生物传感器、微流控芯片、纳米科学与技术以及电化学检测等技术联用，开展用于现场快速检测的高灵敏、高特异、简便的多肿瘤标志物定量检测新方法研究。重点进行电化学免疫传感器制备、便携仪表研制、系统集成及其临床应用验证等研究，实现免疫分析的集成化和微型化，深入分析响应机理，并探索该传感器用于肺癌早期诊断的可行性；进而建立用于多肿瘤标志物快速检测的免疫传感器新理论和新方法，为肺癌早期诊断相关研究提供传感器理论和技术支撑，促进生物医学电子学、信息科学等多学科领域联合发展。

中文关键词： 微流控技术；石墨烯基纳米复合材料；电化学免疫传感器；肿瘤标志物；肺癌早期诊断

英文摘要： Combined detection of multiple cancer markers in the early diagnosis of lung cancer has important significance and clinical needs. However, the common analysis methods in the clinic are not conducive to clinical application of multi -site rapid detection indicators for relying on large equipment and complicated operation. In this project, CEA, CYFRA21-1, NSE and ProGRP are chosen as the target for research starting point according to the suggestions from the American Society of Clinical Biochemistry. Using the integration of such technologies as microfluidic, nanoscience and electrochemical detection, a novel immune sensing technology with high sensitivity and specificity is investigated for rapid detection of multiple lung cancer markers. Studies will be focused on the preparation of the multi-parameter paper-based microfluidic, the development of portable instrumentations, the integration of the systems and their clinical applications of verification. The in-depth analysis of the response mechanism will be included and the feasibility of biosensors for the early diagnosis and the assessment of therapeutic effecacy of the lung cancer will be explored. New theories are further established about the detection of multiple lung cancer markers using the paper-based microfluidic for rapid detection of lung cancer markers. This project could provide novel methods and technical sensor support for the research of the diagnosis and treatment of lung cancer, and promote the breakthrough of frontier technology about the combined rapid immunoassay in the field of medical detection. The implementation of this project will promote cooperation and resource sharing between biomedical and electronics research fields.

英文关键词： Microfluidics;Graphene nanocomposites;Electrochemical immunosensor;Cancer biomarkers;Early diagnosis of lung cancer