结合微流控和磁性分离技术的肺癌标志物SERS生物芯片研究

项目名称： 结合微流控和磁性分离技术的肺癌标志物SERS生物芯片研究

项目编号： No.61302027

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 无线电电子学、电信技术

项目作者： 宋春元

作者单位： 南京邮电大学

项目金额： 27万元

中文摘要： 肺癌已占据我国恶性肿瘤发病率和死亡率第一位，成为我国居民的一大健康杀手。早期诊断是提高肺癌存活率的重要措施。基于表面增强拉曼散射的纳米生物传感技术是实现肿瘤早期检测潜在的灵敏、可靠分析方法。本项目立足于肺癌早期诊断的重大需求，开发基于SERS技术、面向肺癌标志物检测的高灵敏免疫传感器，并结合微流控芯片和磁性分离与富集技术，对血清中的痕量肺癌标志物开展高灵敏、特异性的联合检测研究，建立集样品处理、分析于一体的SERS检测微全分析系统，借助SERS免疫检测技术监视蛋白标志物在血清中的水平来评估疾病潜在的威胁，对肺癌的发生开展早期检测。通过本课题的研究，为SERS技术在肺癌以及其他肿瘤疾病早期诊断中的应用建立技术平台, 也为进一步开发便携的、现场实时（POC）检测设备，实现便捷、高效的肺癌早期筛查做技术准备。

中文关键词： 生物传感器；表面增强拉曼散射；磁性分离；生物芯片；联合检测

英文摘要： In China, lung cancer has occupied the first place of incidence and mortality of malignant tumour, and become the major health killer to the residents. Early diagnosis is an important measure to improve lung cancer survival. Surface-enhanced Raman scattering (SERS)-based biosensing technology is known as a potential, sensitive and reliable analytical techniques to achieve early detection of tumor. Aiming to the significant demand for the early detection of lung cancer, this project is committed to develope highly sensitive SERS-sensors, and combine with the microfluidic chip, as well as magnetic separation and enrichment, to perform highly sensitive, specific joint detection of the trace protein markers of lung cancer in serum, establishing a SERS-based micro total analysis system for sample treatments and analysis. By monitoring the markers and concentrations in serum, medical staffs can assess the disease potential threat and launch the early diagnosis of cancer. Through the study of this subject, a technology platform can be created for the application of the SERS in the early detection of lung cancer, as well as other tumor diseases, which can make technical preparations for the further development of portable device for point-of-care testing of early lung cancer.

英文关键词： Biosensor；surface-enhanced Raman scattering；Magnetic separation；Bio-chip；Joint-detection