纳米金可视化快速检测五氟磺草胺农药残留研究

项目名称： 纳米金可视化快速检测五氟磺草胺农药残留研究

项目编号： No.31501667

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 袁龙飞

作者单位： 中国科学院动物研究所

项目金额： 19万元

中文摘要： 近年来基于表面功能化设计的纳米金可视化检测方法，因其操作简单、灵敏准确以及结果可视等优点，引起了人们越来越多地关注。五氟磺草胺作为水稻田用除草剂中杀草谱最广的农药品种，在全球范围内被广泛使用。然而，迄今为止尚无针对五氟磺草胺或其它农药分子的纳米金可视化检测方法，发展纳米金可视化快速检测农药残留仍然是一个巨大的挑战。本项目拟设计一种纳米金可视化快速检测五氟磺草胺农药残留方法，通过五氟磺草胺中碳氟键、磺酰基、三唑环等典型的吸电子基团与表面修饰三聚氰胺的纳米金粒子上给电子基团氨基之间氢键相互作用，使纳米金的状态从分散变到聚集，同时颜色发生变化，从而实现快速可视化检测。这种方法具有制备简单、成本低廉、容易保存、直观灵敏等优点。成功建立这种新型的纳米金可视化快速检测方法，不但可以为五氟磺草胺农药残留的现场快速检测提供有益的方法参考，并且可为其他农药残留的可视化快速检测提供新的借鉴和思路。

中文关键词： 农药残留；纳米金；可视化；快速检测；五氟磺草胺

英文摘要： In recent years, the colorimetric detection using gold nanoparticles (AuNPs) based on design of unique surface functional visual sensor has aroused more and more attention. Penoxsulam, as a new acetolactate synthase inhibitor herbicide, can be widely used for effective control of several weeds in rice cultivation. However, up to now there are few reports on the colorimetric detection of penoxsulam or other pesticide residues using AuNPs. Development of the colorimetric detection of penoxsulam residue by AuNPs is still a great challenge. In this research, a highly selective and sensitive method is developed for colorimetric detection of penoxsulam residue using AuNPs functionalized with melamine. Considering that penoxsulam has electron withdrawing groups (carbon-fluorine bonds, sulfonyl group, triazole groups) and melamine has electron donating groups (amino groups), we assume that there may be some special hydrogen bonding interactions between penoxsulam and AuNPs functionalized with melamine. Therefore, the presence of the penoxsulam could induce the aggregation of AuNPs through hydrogen-bonding interaction, resulting in a color change from ruby red to royal purple. The distinct change in color and optical properties even could be monitored by naked eyes. The proposed method is convenient, low-cost and highly sensitivity. This work would be helpful for the rapid and on-site detection of penoxsulam or other pesticide residues.

英文关键词： pesticide residues;gold nanoparticles;colorimetric detection;rapid detection;penoxsulam