利用微纳光纤对微流控芯片中的微颗粒和生物细胞的光泳操控研究

项目名称： 利用微纳光纤对微流控芯片中的微颗粒和生物细胞的光泳操控研究

项目编号： No.61205165

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 信息四处

项目作者： 雷宏香

作者单位： 中山大学

项目金额： 29万元

中文摘要： 对微流通道中的微颗粒和生物细胞等的筛选以及分离研究是目前微纳光子学、生物光子学、生命科学、医学等交叉学科领域的前沿研究热点。本项目拟通过研究不同直径的微纳光纤在液体中的导光特性，以及受光照后的微颗粒和生物细胞在液体中的光场分布及热分布情况，研究发生正向和逆向光泳的条件以及光泳力的作用范围。以此为基础设计和制作功能性微流控芯片，将微纳光纤嵌入该芯片中，借助于微纳光纤泄露光场产生的光泳力实现对微流控芯片中微颗粒和生物细胞的有效筛选与连续分离操控。该方法具有自由灵活、无损伤等优点。其结果将在药物筛选、病毒/恶性细胞的有效清除等的进一步研究方面具有重要的学术价值和应用前景。

中文关键词： 微纳光纤；光泳操控；微纳米结构物体和生物细胞；筛选；分离

英文摘要： Sieving and sorting of micro-particles and cells in microfluidic channel are hot topics in the fields of micro-/nano-photonics, biological photonics, life science and medicine. With numerical simulations, we firstly study the optical field distribution of the micro-/nano-fiber and the irradiated micro-/nano-particles in liquid to analyse the proper condition for producting obvious positive/negative photophoresis at a low optical power. Based on the simulation and analysis, we design and fabricate the microfluidic chip to meet the demand of different functions. Then the device will be assembled by embedding the micro-/nano-fiber into the chip by a fiber-positioner. Because of the strong positive/negative photophoretic force induced by the light leaked from the micro-/nano-fiber, we can achieve the effective sieving and continuous sorting of micro-particles and cells on the microfluidic chip with low optical power, less damage and non-contact manipulation. In addition, we will further study the mechanism of photophoretic manipulation for different micro-particles/cells in different liquid environment by changing the wavelength and optical power of light source. The photophoretic manipulation method based on microfluidic chip and micro-/nano-fiber can be further applied in drug screening, removal of virus and ma

英文关键词： micro-/nano-fiber；photophoretic manipulation；miro/nanostructures and cells；sieving；sorting