由表面声波驱动的生物样品制备和分析微系统

项目名称： 由表面声波驱动的生物样品制备和分析微系统

项目编号： No.61274037

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 无线电电子学、电信技术

项目作者： 骆季奎

作者单位： 浙江大学

项目金额： 80万元

中文摘要： 高灵敏度生物传感器可用来检测和诊断各种早期疾病，流行病等，为早日介入 治疗和预防提供可靠的依据，它是一种具有很大应用前景的微纳器件。各国都在加大投入， 开发相关的技术以占据科技和产业的制高点。生物传感器的传感对象主要是细胞内的蛋白质 或 DNA 等，而这些物质被细胞膜所包围，不能直接用于检测。生物取样必须将细胞粉碎，取出细胞物质。本研究独创性的提出了基于表面声波（SAW）的微型细胞粉碎器和 DNA 倍放器 PCR，并将它们与申请人已有的 SAW 微流控技术集成，形成集成生物芯片。利用声波传输液体，由它所产生的声致微流的强大离心力使细胞与微结构碰撞而裂解；并利用声波作加热器、驱动器来倍放 DNA。由于各器件都由表面声波驱动，具有共用性，结构，工艺简单等众多优点，有广泛的应用前景。通过本项目开发出新型的细胞取样器件和 PCR，为下一步开法有自主知识产权的集成芯片大号基础。

中文关键词： 声表面波；生物检测系统；细胞粉碎器；细胞分离；传感器

英文摘要： Biosensors and healthcare devices are types of devices which can detect various diseases and cancers in early stage, hence allow taking measures to prevent and cure them before macrosymptoms appear. Owing to its great importance in science and research,healthcare,drug development,biotechnology and future industry,governments around the world,especially the industrialized nations, have heavily invested in this area, hopefully to become the leading nation in the relevant technologies. Biosensors perform detection through reaction between the probe molecules (Biomarkers) and target molecules which are typically proteins and DNAs. In order to perform the detection,these biological entities must be extracted from the cells, a process called cell lyses.If the detection is based on DNA, then the DNA must be amplified to a certain quantity for analysis, a process called polymerase chain reaction (PCR). Current cell lyses are carried out by mechanical milling, ultrasonic destruction or chemical treatment in a large quantity, not suitable for modern micro-analysis. Furthermore the chemical treatment destroys cell's integrity, unable to be used for detection. These sample preparation processes are time-consuming,label intensive, high cost and potential for cross-contamination, not suitable for on-time, in-field

英文关键词： suface acoustic wave；biodetection system；cell lyses；cell separation；biosensors