拟南芥幼年向成年转变的分子机制研究

项目名称： 拟南芥幼年向成年转变的分子机制研究

项目编号： No.31271313

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 吴刚

作者单位： 浙江农林大学

项目金额： 75万元

中文摘要： 植物幼年向成年转变（植物营养生长过程）是开花的基础。它直接调节植物抗病虫性、抗逆性，调控植物次生代谢产物合成及植物细胞壁组分，调控植物株型等，是一个十分重要的发育过程。前人已对植物从成年向生殖生长阶段转变（即开花）的分子机理进行了深入研究。不过，由于存在表型弱、转变周期长等特点，植物幼年向成年转变过程的研究十分滞后。最近研究表明,miR156是调控植物幼年向成年转变的主要调控因子。miR156通过调控并协调不同SPL（Squamosa promoter binding-like protein）基因的表达来调控该发育进程。然而，miR156是如何受到上游其它因子的调控，不同SPL基因的下游靶基因是什么，这些因子在植物营养生长中的功能是什么还不清楚。本项目将以拟南芥为研究对象，采用正反向遗传学、分子生物学及基因组学的方法研究上述问题，为深入了解植物从幼年向成年转变的分子机理提供基础。

中文关键词： 幼年向成年阶段转变；miR156；突变体；表观遗传；

英文摘要： The juvenile-to-adult transition (i.e. vegetative development) in plants is a paramountly important process partially in that it determines reproductive competence. It directly regulates plant resistance to biotic and abiotic stress, plant secondary compounds synthesis plant cell wall compenents, and plant architecture, etc. The transition of adult-to-reproductive phase (also known as flowering) is a well-studied process because of its obvious phenotypes associated with this transition. While, the mechsnism of the juvenile-to-adult transition remains unclear mainly due to the fact that the phenotypes associated with this developmental process are relatively subtle, and the much longer time it takes to complete this process. Recent research shows that miR156 is the master regulator of vegetative development in plants by regulating and coordinating different SPL (squamoso promoter binding-like proteins) genes. However, how miR156 itself is regulated by upstream external factors, what is the identity of the SPL targets remain elusive. We are very much interested in identifying these factors controlling miR156 expression and the targets of SPL proteins, as well as understanding their roles in vegetative development in Arabidopsis by using forward and reverse genetics, molecular biology and genomic approaches. The re

英文关键词： Juvenile-to-Adult phase transition；miR156；mutant；epigenetic regulation；