基于人诱导性多能干细胞技术的长QT综合征III型发病机制及药物筛查研究

项目名称： 基于人诱导性多能干细胞技术的长QT综合征III型发病机制及药物筛查研究

项目编号： No.81470452

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 张艳敏

作者单位： 西安交通大学

项目金额： 73万元

中文摘要： 遗传性长QT综合征III型（LQT3）的致病基因SCN5A编码心脏钠通道， 建立患者突变特异性体外细胞模型是实现个性化医疗的关键。本研究在前期基因测序确定一个LQT3家系SCN5A-A1180V突变基础上，通过体细胞重编程，将Oct3/4、Sox2、LANOG 和 LIN28四种转录因子基因克隆入慢病毒载体，引入患者体细胞，通过生物学鉴定，建立LQT3患者SCN5A-A1180V突变特异性诱导多能干细胞(iPSC)细胞系；采用心肌细胞定向分化技术，将iPSC分化为功能性心肌细胞（iPSC-CM），建立LQT3患者突变特异体外细胞模型；运用膜片钳、激光共聚焦、分子生物学、多导电极阵列（MEA）技术研究SCN5A-A1180V-iPSC-CM心律失常发病机制，进行抗心律失常药物筛选，发现延长和缩短患者心肌细胞动作电位的药物，建立数学仿真计算机模型，探索遗传性心律失常个性化治疗新途径。

中文关键词： 离子通道；心律失常；长QT综合征；诱导多能干细胞；心肌细胞

英文摘要： Inherited long QT syndromes (LQTs), characterized by electrocardiographic (ECG) QT interval prolongation and episodes of ventricular tachycardia and fibrillation, are major causes of sudden cardiac death in children and young adults. Thirteen genes are associated with LQTS. SCN5A encoding cardiac sodium channels is the causative gene underlying LQT type 3 (LQT3). The consequences of SCN5A mutations are patient-specific. Different mutation positions and functional features produce a phenotypic spectrum ranging from being asymptomatic to causing potentially fatal arrhythmias. Development of disease cell models specific to the patient's mutation leading to personalized treatment is a current focus of research. The present proposal is based on a recently reported LQT3-related SCN5A-A1180V mutation. It will use somatic reprogramming techniques to develop LQT3 patient SCN5A-A1180V mutation-specific induced pluripotent stem cell (iPSC) by introducing Oct3/4,Sox2, LANOG and LIN28 into patient dermal fibroblasts. iPSCs are further differentiated into cardiomyocytes (iPSC-CM) by co-culturing iPSCs with EDN-2. The electrophysiological characteristics and expression profiles of SCN5A-A1180V-iPSC-CM cell models including action potential, ion current and gene/protein expression characteristics will be tested using patch clamp, laser focus microscopy, and molecular biology techniques. Drug screen investigation will be performed using high throughput patch clamp and multi electrode arrays to identify shortened or prolonged QT interval and the actions of candidate drugs. Computational modeling will be developed using the electrophysiological and biological results. The proposal would benefit the future development of personalized medicine in the management of inherited arrhythmia patients.

英文关键词： Ion channel;Arrhythmia;Long QT syndrome;Induced pluripotent stem cell;cardiomyocyte