果蝇CG12765基因功能和分子机制的研究

项目名称： 果蝇CG12765基因功能和分子机制的研究

项目编号： No.30871342

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 电工技术

项目作者： 俞海菁

作者单位： 云南大学

项目金额： 35万元

中文摘要： 人类新基因c1orf37-dup的果蝇同源基因CG12765的缺失导致果蝇温度敏感型致死。表型分析显示该基因缺失影响蜕皮激素指导的生长发育通路。在限制性温度29 C生长的果蝇个体中，蜕皮激素缺乏正常发育中呈现的浓度峰值，导致个体在蜕皮和化蛹、变态时期死亡。功能分析显示CG12765基因不参与蜕皮激素的化学合成过程。针对个体变态过程的基因表达分析，我们发现CG12765基因缺失严重影响由蜕皮激素指导的、受精密调控的基因层次表达系统。定量分析显示其多个功能组的基因表达谱发生了巨大变化；而同一功能组的基因之间，其表达的变化程度存在差异，暗示正常发育中蜕皮激素诱导的基因层次表达与蜕皮激素浓度间存在复杂的反馈调控关系。蛋白组学研究显示昆虫变态期能量储备相关蛋白FBP1(fat body protein 1)的表达量显著下降，或许是个体致死的直接原因。CG12765基因功能的阐明有助于探索人类进化中未知功能新基因c1orf37-dup起源产生后所发挥的作用。

中文关键词： CG12765基因；基因功能；蜕皮激素通路；基因表达调控

英文摘要： The Drosophila CG12765 gene is the homologous gene of the human-specific new gene c1orf37-dup. The CG12765-deficiency results in temperature-sensitive lethality in fruit flies. The phenotypic analyses revealed that this gene influences fly lethality through ecdysone-induced developmental pathway. At the restrictive temperature 29 C, the CG12765-deficient flies lack the normal ecdysone pulses presenting in the wild-type controls, which results in individual death during ecdysis and metamorphosis. The functional assay demonstrated that this gene is not involved in the chemical synthesis of ecdysone. During metamorphosis, the elegantly regulated gene expression hierarchy directed by ecdysone is severely damaged in the deficient flies. The significant changes in the expression profile were disclosed by the quantitative analyses on several functional classes of genes. However, in the same functional class, discrepancy in the pattern of expression changes was observed among the genes. These findings suggested that through development, there is the subtle and complicated feedback regulation between the ecdysone-induced gene expression hierarchy and the ecdysone level. Proteomic analysis showed that the amount of the fat body protein 1, a highly expressed protein around metamorphosis for energy preparation, is dramatically dropped in the deficient flies, which might be the direct cause of the lethal phenotype. The understanding of the CG12765 function is helpful for exploring the contribution of the functionally unknown new human-specific gene c1orf37-dup in human evolution.

英文关键词： CG12765; gene function; ecdysone pathway; gene expression regulation