经颅磁刺激对帕金森病运动功能及突触可塑性的影响及机制研究

项目名称： 经颅磁刺激对帕金森病运动功能及突触可塑性的影响及机制研究

项目编号： No.81200996

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 神经系统疾病、精神疾病

项目作者： 李玥

作者单位： 中国科学院电工研究所

项目金额： 23万元

中文摘要： 帕金森病(Parkinson's disease, PD)是最常见的慢性神经变性性疾病之一，目前临床尚无有效的治疗方法。已有很多研究证实经颅磁刺激能改善PD的运动障碍，然而由于机制不清限制了其在临床中的应用。突触可塑性是神经损伤和修复的神经生物学基础，在PD恢复中扮演重要角色。本项目拟分别采用高频和低频经颅磁刺激观察经颅磁刺激对帕金森病大鼠运动功能的改善作用，利用电镜、激光共聚焦显微镜、免疫荧光、western blot等技术研究经颅磁刺激后纹状体突触数、突触超微结构改变以及神经元树突生长和突触发生的变化，并分析相关突触蛋白表达，同时利用特异性拮抗剂干预，分析经颅磁刺激影响突触可塑性的分子机制，为推动经颅磁刺激在临床的应用提供理论基础。

中文关键词： 经颅磁刺激；突触可塑性；NMDA受体复合物；；

英文摘要： Parkinson's disease（PD）is a common neurodegenerative disorder with no clinically efficient remedy available at the moment. Many studies have confirmed that transcranial magnetic stimulation (TMS) can improve movement disorders of PD. However, its application in clinical practice is limited due to the mechanism is unclear. Synaptic plasticity plays a important role in nerve injury and repair. In PD, there is a pathological synaptic plasticity and modulation of cortico-striatal synaptic plasticity might prove useful in the treatment of motor symptoms. In this project the effect of high-frequency and low-frequency transcranial magnetic stimulation on the motor function of Parkinson's disease model are studied. Synapse number、synapse ultrastructure and synaptogenesis are detected by electron microscopy、confocal laser scanning microscopy、immunofluorescence、Western blot. The relationship between N-methyl-D-aspartate (NMDA) receptor signaling complex and transcranial magnetic stimulation is also studied. Meanwhile, we also investigate the role of NMDAR antagonist in order to explain the mechanism. Our study will provide a theoretical basis for the clinical applications of the transcranial magnetic stimulation.

英文关键词： transcranial magnetic stimulation；synaptic plasticity；NMDA receptor signaling complex；；