促性腺激素调节C-型钠肽及其受体表达的分子机理

项目名称： 促性腺激素调节C-型钠肽及其受体表达的分子机理

项目编号： No.31272523

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 张美佳

作者单位： 中国农业大学

项目金额： 80万元

中文摘要： C-型钠肽（CNP/NPPC）及其受体NPR2维持卵母细胞减数分裂的阻滞,其在FSH促进卵泡发育时高表达,而在LH诱导卵母细胞成熟和排卵时低表达。但是其表达调节的分子机理还不清楚。本项目以已取得的研究成果和最新的研究进展为依据，应用体外培养卵母细胞和卵泡，以及基因突变小鼠为模型，主要研究促性腺激素、性激素、旁分泌因子和转录因子对CNP及其受体在卵巢中表达调节的分子机理；CNP及其受体功能性变化与促性腺激素诱导卵母细胞成熟之间的相关性；CNP及其受体的表达与卵泡中cGMP水平变化之间的关系。研究结果可进一步了解FSH和LH在卵泡发育与卵母细胞成熟过程中的不同作用、性激素在卵母细胞成熟中的作用机理，补充和完善卵母细胞成熟理论，对进一步完善促性腺激素诱导卵母细胞成熟中的分子机制、阐明多囊卵巢综合征的发病机理具有重要的理论和实际意义。

中文关键词： C-型钠肽；促卵泡素；促黄体素；雌激素；钙离子

英文摘要： Fully grown mammalian oocytes in Graafian follicles are maintained in meiotic prophase arrest until the preovulatory surge of luteinizing hormone (LH) triggers the resumption of meiosis and ovulation. The mature oocytes (eggs) are then available for fertilization within the oviduct. The granulosa cell ligand C-type natriuretic peptide (CNP/NPPC) and its receptor NPR2 in cumulus cells prevent precocious meiotic maturation, which is critical for maturation and ovulation synchrony and for normal female fertility. Further study shows that FSH down-regulates, but LH up-regulates their expression. However, the molecular mechanism is not clear. The objective of the present project is to study the mechanism of reproductive hormones (FSH,LH), sex hormones, transcription factor, oocyte-derived paracrine factors on the expression of CNP and NPR2 in mouse follicles, their role in LH-induced oocyte maturation, and the relationship with cGMP levels in the follicle. These results will further understand the mechanism of FSH-induced follicular development and LH-induced oocyte maturation, reinforce the theory of mammalian oocyte maturation, and provide foundation for the pathogenesis of polycystic ovary syndrome.

英文关键词： NPPC；FSH；LH；E2；Calcium