肝细胞癌Gd-EOB-DTPA增强MR成像肝胆期信号的分子病理机制及对恶性程度与预后评估的研究

项目名称： 肝细胞癌Gd-EOB-DTPA增强MR成像肝胆期信号的分子病理机制及对恶性程度与预后评估的研究

项目编号： No.81471658

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 宋彬

作者单位： 四川大学

项目金额： 80万元

中文摘要： 肝细胞癌（Hepatocellular carcinoma，HCC）是临床上最常见的恶性肿瘤之一，准确评价HCC的恶性程度，对治疗方案的制定及评估预后非常关键。本研究前瞻性纳入拟行外科切除手术的肝癌患者,在术前、术后随访行Gd-EOB-DTPA增强MR检查。对肿瘤标本行免疫荧光、蛋白质印迹法及聚合酶链式反应检查，检测肝细胞表面阴离子转运多肽（OATP）、多耐药相关蛋白（MRP）及肝细胞核因子3β（HNF-3β）表达情况。建立OATP8、MRP、HNF-3β等分子水平指标与肝癌恶性程度的关系，再以此为基础，探索肝细胞癌Gd-EOB-DTPA增强MR肝胆期成像与其恶性程度及预后的关系,并阐明其分子病理学机制。

中文关键词： 磁共振成像；对比剂；Gd-EOB-DTPA；肝细胞癌

英文摘要： Hepatocellular carcinoma (HCC) is one of the most common malignancies in clinic. Malignancy evaluation of HCC is critical for treatment and prognosis. This study prospectively includes patients with HCC undergoing surgery，Gd-EOB-DTPA-enhanced MR examination in preoperative stage and regular follow-up are performed. Immunofluorescence and westernd polymerase chain reaction are performed for tumor specimens to analyze the expression of anion transportingn blotting a polypeptide(OATP), multi-drug resistance associated protein(MRP)and hepatocyte nuclear factor 3β(HNF-3β).Evaluate the relationship between OATP, MRP, HNF-3βand differentiated degree, using which can study the relationship between Gd-EOB-DTPA-enhanced MR imaging findings of HCC and malignancy degree and prognosis，and clarify the molecular mechanism.

英文关键词： magnetic resonance imaging;contrast agent;gadoxetic acid;hepatocellular carcinoma