松茸多糖TMP-A免疫调节活性及其相关分子机制的研究

项目名称： 松茸多糖TMP-A免疫调节活性及其相关分子机制的研究

项目编号： No.31200012

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 微生物学、植物学

项目作者： 丁祥

作者单位： 西华师范大学

项目金额： 23万元

中文摘要： 真菌多糖多具有抗氧化、抗肿瘤、抗病毒和免疫调节等生物学活性，但由于多糖分离纯化困难及其结构复杂，能够得到结晶单体、阐明其结构、具有免疫调节活性且分子机制明确的多糖匮乏。本研究拟以前期研究中获得的松茸多糖结晶单体TMP-A为研究对象，运用细胞生物学技术研究TMP-A对肝细胞和脾细胞的增殖效应以及对巨噬细胞的吞噬效应的影响，结合荷瘤S180小鼠免疫实验，研究TMP-A的免疫活性。通过运用基因芯片、荧光定量PCR技术、酶联免疫实验和免疫印迹技术，研究其对肿瘤坏死因子（TNF-a）、白细胞介素（IL-2、IL-6）、干扰素(IFN)、一氧化氮合酶（iNOS）和集落刺激因子（CSF）等免疫基因转录和翻译水平的效应，以探寻TMP-A免疫活性及其相关分子机制，为真菌多糖的研究开发提供相关科学依据。

中文关键词： 真菌；松茸；多糖；结构解析；免疫调节

英文摘要： Polysaccharides are long carbohydrate molecules, of repeating units joined together by glycosidic bonds. They are often linear, but may also be even highly branched. Polysaccharides are often quite heterogeneous, containing slight modifications of the repeating unit. Depending on the structure, these macromolecules can have distinct properties from their monosaccharide building blocks. They may be amorphous or even insoluble in water . Polysaccharides known as "biological response modulators",reflects its significant immunomodulatory activity, such as promoting the proliferation and differentiation of immune cells, activated T cells and B cells, secrete a variety of lymphokines, regulate the balance of nerve - endocrine - immune regulatory network, and promote the DNA, RNA, protein synthesis et al . In recent years, as more and more polysaccharides have been reported to exhibit a variety of biological activities, including anti-tumor, immunostimulation, anti-oxidation etc., the nonstarch polysaccharides have emerged as an important class of bioactive natural products. In many oriental countries, several immunoceuticals composed of polysaccharides have been accepted such as lentinan, schizophyllan and krestin.Cancer, known medically as a malignant neoplasm, is a term for a large group of different diseases, all i

英文关键词： Fungus；Tricholoma matsutake；Polysaccharide；Structure elucidation；Immunomodulatory