基于同步辐射荧光成像技术研究人三阴性乳腺癌细胞异常铁代谢调控机制

项目名称： 基于同步辐射荧光成像技术研究人三阴性乳腺癌细胞异常铁代谢调控机制

项目编号： No.U1532116

项目类型： 联合基金项目

立项/批准年度： 2016

项目学科： 数理科学和化学

项目作者： 刘苹

作者单位： 上海交通大学

项目金额： 56万元

中文摘要： 三阴性乳腺癌由于其发病率高、局部复发和远端高转移，受到重视。我们前期工作在上海光源应用同步辐射荧光成像技术研究铁在人和鼠源三阴性乳腺癌组织的分布;以及铁与三阴性人乳腺癌细胞迁移的关系，提示铁参与了三阴性乳腺癌的恶性发展，同时发现三阴性乳腺癌细胞较侵袭性弱的乳腺癌细胞具有不同的铁代谢水平，但三阴性乳腺癌细胞铁代谢机制尚不清楚。本研究将应用同步辐射荧光成像技术具有高分辨率、高灵敏度、直观显示多种元素分布的优势，建立同步辐射X射线荧光成像技术亚细胞定位元素与蛋白分布的方法；探索三阴性乳腺癌细胞铁摄取途径、细胞内铁转运、储存及利用规律；阐明三阴性乳腺癌细胞铁代谢调控机制。我们的研究将为发展新型乳腺癌治疗策略提供理论基础,进一步推动同步辐射影像的医学应用研究。

中文关键词： 同步辐射荧光成像；人三阴性乳腺癌细胞；铁代谢；调控机制

英文摘要： Due to its high incidence, high local recurrence and distal metastasis, significant attention has been paid in three negative breast cancer. Our previous works studied the distribution of iron in human and mouse source three negative breast cancer tissue using synchrotron radiation fluorescence imaging at SSRF; and the relationship between iron and migration of human three negative breast cancer cells. The results indicated that iron was involved in malignant development of three negative breast cancer. At the same time, we found that three negative breast cancer cells had different levels of iron metabolism compared to more weak invasive breast cancer cells, but the regulatory mechanism of iron metabolism in three negative breast cancer cells is not clear. This study will apply synchrotron radiation fluorescence imaging with the advantage of high resolution, high sensitivity and visual display of multi-elements distribution in the establishment of imaging method for subcellular localization of protein and elements distribution; exploration of iron uptake pathway, iron transport, storage and utilization in three negative breast cancer cells; elucidating the regulatory mechanism of iron metabolism in three negative breast cancer cells. Our study will provide the theory basis of the development of novel breast cancer treatment strategies, further promote the research on medical application of synchrotron radiation imaging.

英文关键词： synchrotron radiation fluorescence imaging;human three negative breast cancer cells ; iron metabolism ;regulatory mechanism