箭叶淫羊藿叶片发育过程中花青素苷和黄酮醇苷代谢分支的分子调控

项目名称： 箭叶淫羊藿叶片发育过程中花青素苷和黄酮醇苷代谢分支的分子调控

项目编号： No.31270340

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 王瑛

作者单位： 中国科学院武汉植物园

项目金额： 82万元

中文摘要： 传统中药材淫羊藿中主要的活性成分淫羊藿苷等因其独特的结构和功能活性，已经被单独列为一类重要的黄酮醇苷。一方面由于过渡采收野生淫羊藿造成中药材资源急剧减少，另一方面随着抗癌、治哮喘等新功能的发掘国内外对淫羊藿的需求不断增加。因此，急需深入研究淫羊藿苷的合成和分子调控机制，为淫羊藿黄酮醇苷的生物合成、淫羊藿的分子育种、人工栽培奠定重要的理论基础。本项目通过箭叶淫羊藿叶片转录组测序、差减文库、同源克隆等方法克隆类黄酮代谢途径的主要结构基因和调控因子，重点解析下游花青素苷和黄酮醇苷的代谢分支；通过分析幼嫩红色叶片到成熟绿色叶片发育过程中基因表达和化学成分积累的关系，鉴定花青素苷和黄酮醇苷两个代谢分支的重要结构基因，及发掘调控这两个代谢分支的转录因子；研究转录因子的基因功能、与结构基因启动子的互作、不同转录因子之间的互作，解析淫羊藿中类黄酮代谢下游的花青素苷和黄酮醇苷代谢分支的关系和分子调控机制。

中文关键词： 淫羊藿；转录因子；黄酮醇代谢；花青苷代谢；基因功能

英文摘要： Because of its unique structural and functional activity, the main active ingredient icariin in traditional Chinese medicine herba epimedii (prepared from arial parts of Epimedium species) has been singled out as an important class of flavonol glycosides. On the one hand, Epimedium wild resources reduced dramatically due to the over harvesting in the past decade, on the other hand, the domestic and foreign demand of herba epimedii has been increasing considerably with the discovery of new functions (such as anticancer, the treatment of asthma and other new features). Therefore, in-depth study of icariin biosynthesis and molecular control mechanism is in urgent need to lay an important theoretical basis for the biosynthesis of flavonol glycosides in Epimedium species, as well as the molecular breeding and cultivation. Combining leaf transcriptome sequencing, suppression subtractive hybridization library, and homologous cloning method, the genes including structural genes and regulatory factors relating to the flavonoid biosynthetic pathway will be cloned, especially those for the anthocyanin and flavonol glycosides branches. The relationship between gene expression and accumulation of chemical composition will be explored during the leaf development from young red leaves to mature green leaves. The function of tr

英文关键词： Epimedium；transcription factor；flavonol biosynthesis；anthocyanin biosynthesis；gene function