CO/HO-1及NO信号系统介导5-氨基乙酰丙酸(ALA)提高拟南芥盐适应性机理研究

项目名称： CO/HO-1及NO信号系统介导5-氨基乙酰丙酸(ALA)提高拟南芥盐适应性机理研究

项目编号： No.31300222

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 张春平

作者单位： 徐州医学院

项目金额： 22万元

中文摘要： ALA作为一种重要的植物内源物质，在植物抗盐胁迫方面具有重要作用，但目前对ALA的具体抗盐机理尚不明确，没有形成相关的ALA抗盐胁迫信号通路。ALA在植物体内直接参与卟啉类化合物的生物合成，进而生成血红素，血红素作为底物在HO-1的作用下发生分解并生成具有重要信号传导作用的CO。研究表明CO与NO信号分子具有相似的生理功能，并且在的抗盐胁迫中具有显著地作用。本项目基于对ALA、CO及NO抗盐作用所做的前期研究，以拟南芥种子及幼苗为材料，将ALA在盐胁迫下的代谢及信号转导通过血红素的代谢与CO/HO及NO相关联。采取对ALA代谢过程中不同的关键酶进行抑制或诱导的方法，阻断或诱导通路，同时利用药理学及分子生物学手段，在生理生化及分子生物学水平上，明确ALA信号转导途径及CO/HO和NO在信号通路中的介导作用，从而揭示ALA提高拟南芥盐适应性的机理，此研究将为药用植物抗逆提供理论依据和技术支撑。

中文关键词： 拟南芥；5-氨基乙酰丙酸；抗盐机理；一氧化碳；一氧化氮

英文摘要： 5-Aminolevulinic acid (ALA) as a kind of important plant endogenous substance, has an significant role in plant resistance to salinity stress. However, the specific mechanism of ALA to salinity is not clear at present, and there is no formation of related salinity resistance signaling pathway to ALA now. ALA is a key precursor in the biosynthesis of all porphyrins compounds, such as Chlorophyll, heme, and phytochrome. Heme as a substrate to decompose and generate carbon monoxide (CO) under the action of HO-1, which has an important signaling role in salinity stress resistance. Studies show that the CO and nitric oxide (NO) signaling molecules have similar physiological functions, and has a significant role in the resistance to salinity stress. The project based on previous studies of ALA, CO and NO to salinity stress resistance, use the seeds and seedlings of Arabidopsis thaliana as experimental materials, correlate the ALA metabolism and signal transduction under salinity stress by the heme metabolism, CO/HO, and NO. Taken the methods of inhibition or induction of key enzymes in ALA metabolic processes to block or induce the signaling channel. Take advantage of the pharmacology and molecular biology methods, at the levels of the physiological, biochemical and molecular biological, make clear the ALA signal tran

英文关键词： Arabidopsis thaliana；Salt resistant mechanism；5-Aminolevulinic acid；Carbon monoxide；Nitric oxide