弓形虫酪蛋白激酶CK1对细胞周期的调控

项目名称： 弓形虫酪蛋白激酶CK1对细胞周期的调控

项目编号： No.31472183

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 水产学、兽医学

项目作者： 魏峰

作者单位： 吉林农业大学

项目金额： 88万元

中文摘要： 弓形虫病是一种重要的人兽共患寄生虫病，严重危害人类健康、食品安全和畜牧业生产，目前仍无理想的防治方法。酪蛋白激酶（CK）是一类保守的丝氨酸／苏氨酸蛋白激酶，参与囊泡转运、DNA修复、细胞周期和细胞分裂等生命过程。弓形虫编码CK1α和CK1β基因，其功能还未见报道。我们通过敲除弓形虫CK1α，发现其与虫体细胞周期相关，但其作用机制尚不清楚。本研究拟在前期研究结果基础上，确定弓形虫CK1α和和CK1β的表达特性；构建弓形虫CK1α和CK1β缺失株，通过CK1α和CK1β缺失株与野生株转录组测序及差异磷酸化蛋白的比较，确定弓形虫CK1α和CK1β对细胞周期的调控及机制，为弓形虫基因缺失疫苗及CK1靶向药物研制奠定基础。

中文关键词： 弓形虫；细胞周期；酪蛋白激酶1

英文摘要： Toxoplasmiasis, caused by the protozoan Toxoplasma gondii, is one of the important zoonotic diseases, which have seriously affected human health, food safety and animal production in the world. However, there is still no ideal control strategy against the disease. Casein kinase 1 (CK1) belong to a conserved family of serine/threonine protein kinases that plays an important and diverse role in vesicular trafficking, DNA repair, cell cycle progression and cytokinesis in organisms from yeast to humans. In multicellular organisms CK1 enzymes also regulate developmental pathways, control circadian rhythms and have been implicated in Alzheimer's disease progression. T. gondii encodes two CK1 isoforms, whose functions are still unknown. In this study, we will determine the expression characteristics of T. gondii CK1α and CK1β, and construct CK1α- and CK1β-deficient strains. By comparison of transcriptome sequencing and phosphorylated proteins of CK1α- and CK1β-deficient strains and wild type strain GT1, we will determine cell cycle regulation and mechanisms of T. gondii by CK1α- and CK1β, which will provide foundation for development of gene deletion vaccine and drug targeting CK1 for T. gondii.

英文关键词： Toxoplasma;Cell cycle;Casein kinase CK1