马铃薯块茎发育过程中茉莉酸调控的磷酸化蛋白质组研究

项目名称： 马铃薯块茎发育过程中茉莉酸调控的磷酸化蛋白质组研究

项目编号： No.31471433

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 张峰

作者单位： 甘肃农业大学

项目金额： 87万元

中文摘要： 研究马铃薯块茎发育过程，对揭示地下贮藏器官发育机理，阐明马铃薯产量和品质性状的形成具有重要的理论价值和实践意义。本研究以离体块茎发育体系和活体块茎发育体系的块茎为研究对象，运用表观形态学，植物生理学、蛋白质组学、生物信息学和分子生物学等多学科综合手段，对不同发育阶段块茎的茉莉酸（Jasmonic acid,JA）含量变化、性状特征、糖分积累等进行测定；对过量JA和阻断JA合成后不同发育阶段块茎蛋白进行同位素相对和绝对定量(Isobaric tag for relative and absolute quantitation,iTRAQ)标记，质谱分析，功能预测和分类。解析JA诱导的蛋白质磷酸化调节和控制方式，探究JA调控的磷酸化蛋白质组参与块茎发育的机制及模式，确定JA调控马铃薯块茎发育的途径，分离关键的磷酸化蛋白，并对其功能进行验证。这些研究结果将对揭示马铃薯块茎发育机理提供新思路。

中文关键词： 马铃薯；块茎发育；茉莉酸；磷酸化；蛋白质组

英文摘要： Tuber formation involves many important biological processes, including carbon partitioning,signal transduction and meristem determination.Tuber developmental process also requires the comprehensive regulation of many endogenous hormones,which has become an important reaserch field at present.Investigation of the developmental process of tuber in potato could be better understanding of yield and quality underlying tuber formation and development.In this poject, potato tuber development system in vitro and in vivo were used as reaserch object,and the plant physiology,morphology,bioinformatics,molecular biology,iTRAQ (isobaric tags for relative and absolute quantitation) and other multi-disciplinary methods were applied.The relationship between jasmonic acid (JA) content changes and phosphoproteins in tuber development will be analyzed.These proteins will be function prediction and classification. These new critical proteins and their genes will be cloned and role of these proteins and genes involved in the regulation pathway of JA in tuber development will be examined.The results will clarify the function and regulation mechanism of phosphoproteins involved in JA regulating pathway during tuber development, which could provide a theoretical basis for the formation of potato yield and quality traits and will contribute to help us better understanding of physiological mechanisms underlying potato tuber formation and development.

英文关键词： potato;tuber development;jasmonic acid;phosphorylation;proteomics