硫化氢信号通过与生长素和乙烯互作来调节番茄花柄脱落的分子机理

项目名称： 硫化氢信号通过与生长素和乙烯互作来调节番茄花柄脱落的分子机理

项目编号： No.31501772

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 刘旦梅

作者单位： 山西大学

项目金额： 20万元

中文摘要： 器官脱落是植物非常重要的生理过程，更是育种学家关注的重要农艺性状。离区获取响应脱落信号的能力是该生理过程中最为复杂的一步，但分子机理研究相对薄弱。硫化氢（H2S）是近年来发现的气体信号分子，它在植物生长发育、逆境胁迫等多方面发挥重要作用。我们的前期工作表明，H2S处理可明显延迟番茄花柄脱落；显著抑制花柄中细胞壁降解相关基因的表达；还进一步发现花柄中活性生长素的含量也随之发生了剧烈变化。这些有趣的现象强烈暗示了在离区获取响应脱落信号能力的过程中，H2S极可能通过与花柄内源激素互作的方式来行使其重要功能。.本研究拟在前期工作基础上，通过生理生化、分子遗传等研究手段，阐明H2S信号对番茄花柄脱落的影响；分析H2S同生长素和乙烯在番茄花柄脱落中的关系；发掘花柄内源H2S含量控制因子。以期从气体信号分子这一全新视角阐释番茄花柄脱落分子机理，为开发新型的植物器官脱落调节剂提供理论支持。

中文关键词： 番茄；植物激素；信号转导；基因功能；器官生长发育

英文摘要： Abscission is a critical biological process during plant life cycles, and also an important trait with agronomic significance. In the process of abscission, the most complicated step is to acquire the competence to react to abscission signals. However, the molecular mechanism underlying this is still elusive. Hydrogen sulfide (H2S) is a newly found gasotransmitter proven to play multiple roles in plant development and stress resistance. Previously, we found that H2S treatment could significantly retard the abscission of the flower pedicel of tomato, and inhibit the up-regulation of cell wall related genes. Our preliminary analysis also suggested a remarkable increase of the amount of active auxin in the treated pedicels, indicating that H2S might regulate the process of pedicel abscission through the interaction with the endogenous hormones in tomato flower pedicels.. Based on these results, we propose in this project to use physiological, biochemical and genetic means to: (1) clarify the effects of H2S during the process of pedicel abscission; (2) investigate the relationship among H2S, auxin and ethylene in tomato pedicel abscission; (3) explore the upstream regulator of H2S synthase in flower pedicel. This work will elucidate an improved mechanism for the abscission regulation from the angel of gasotranmitter, and provide great insights for a new abscission regulator in plant.

英文关键词： tomato;phytohormone;signal transduction;gene function;organ growth and development