LncRNA在早期断奶仔猪肠道损伤过程中的调控作用

项目名称： LncRNA在早期断奶仔猪肠道损伤过程中的调控作用

项目编号： No.31501966

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 畜牧学与草地科学

项目作者： 刘淑杰

作者单位： 浙江省农业科学院

项目金额： 20万元

中文摘要： 断奶应激造成仔猪肠道损伤是早期断奶技术实施的最大障碍。尽管研究显示早期断奶对肠道形态结构、消化吸收功能及功能蛋白表达等均产生不利影响，然而仔猪肠道损伤所经历的分子作用机制仍不清楚。最新发展的长链非编码RNA（lncRNA）具有从多个水平调控基因表达，参与支配多种信号通路的重要作用，几乎与机体整个生命进程和病理过程密切相关，而lncRNA与仔猪肠道功能关系研究尚未开展。本项目通过高通量测序、基因过表达和沉默等技术，筛选出早期断奶仔猪肠道差异表达的LncRNA，确定具有关键作用的lncRNA及其靶基因，研究lncRNA对靶基因和肠道的调控功能，分析lncRNA在细胞中的富集定位及与其相互作用的蛋白质，揭示LncRNA在肠道损伤过程中的功能调节，以全新的视角探讨断奶仔猪肠道损伤的分子作用机制。该项目顺利实施对解决仔猪早期断奶难题以及全面提升仔猪健康生产具有重要意义。

中文关键词： 长链非编码RNA；早期断奶；仔猪；肠道损伤；调节机制

英文摘要： Weaning stress causes intestinal injury of piglets, which becomes the biggest obstacle of early weaned technology implemented. Studies showed that early weaning had adverse effects on intestinal structure, digestion and absorption function, and expression of functional proteins. However, the molecular mechanism of damage process of intestine remains unclear. Recently developed long noncoding RNAs (lncRNAs) play an important role in the regulation of gene expression and control of molecular signaling pathways, and are relevant to whole life course and pathologic process. The research of lncRNAs function on piglets’ intestine has not been involved. The project filtered differentially expressed lncRNAs of intestine in early-weaned piglets by high-throughput sequencing, the construction of over-expression and interference vectors, and discovered key lncRNAs and theirs target molecules. Furthermore, we studied the regulation of lncRNAs on target molecules and intestine, and analyzed the location of the lncRNAs in the cell and the proteins interacting with the lncRNAs. The project revealed the regulation role of lncRNAs in the course of intestinal damage, and explored the molecular mechanism of intestinal injury in weaned piglets from a totally new perspective. The successful implementation of the project will have great significance to solve the problem of early weaning and improve the health of piglets.

英文关键词： Long non-coding RNA;early weaning;piglets;intestinal structure;regulation mechanism