新型快速成型技术构建的复合植物小分子淫羊藿素的生物支架材料在骨缺损修复中的应用

项目名称： 新型快速成型技术构建的复合植物小分子淫羊藿素的生物支架材料在骨缺损修复中的应用

项目编号： No.51303041

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 一般工业技术

项目作者： 陈诗慧

作者单位： 哈尔滨医科大学

项目金额： 25万元

中文摘要： 各种原因引起的大范围骨缺损(如骨肿瘤、骨质疏松、骨坏死等)仍然是骨科临床较难治愈的病症，为使得缺损部位骨质重建，维持骨长度和保护骨连接仍然是骨外科的一大挑战。具有适当生物力学性能和有效促进成骨活性的骨修复材料对骨缺损的治疗修复尤为关键，此类生物材料的研发具有重要的医学临床意义及社会经济价值。外源性生长因子，植物小分子淫羊藿素(icaritin)具有较稳定的化学结构和较明显的成骨促进作用，利用低温快速成型技术对复合材料的组成、结构和力学参数进行设计和调控，研究个性化、多参数的多孔支架材料的成型方法及其材料学特征。本研究以大鼠颅骨临界骨缺损模型为研究对象，用材料学、生物力学、生物学和影像学等手段对多孔支架材料的力学性能、降解性能和促成骨能力进行考察。从基因、细胞和组织三个层面解释该支架材料的骨缺损修复机理，并为系统评估其作为新型材料修复骨缺损的应用价值奠定了技术基础。

中文关键词： 颅骨缺损；支架材料；3D打印；icaritin；成骨活性

英文摘要： Critical-sized bone defects resulted from various reasons (such as bone tumor, osteoprosis and osteonecrosis) remain critical chanllenges in reconstruction of large bone defects with bone original length and integration. The key points for bone healing enhancement are that bone repair materials should possess the excellent biomechanical properties, osteogenic potentials, and so on, which has remarkable clinical significance and economic value. This project is initated, based on prelimary studies, by a kind of bioactive phytomolecule icaritin served as an exogenous growth factor with stable chemical structure and significant osteogenic activity that could be effectively incorporated into PLGA/TCP composite scaffold materials.A novel porous PLGA/TCP/icaritin scaffold developed by using the low temperature rapid-prototyping technology for bone defect repair based on the established cranial critical-sized defect in rat model. The osteopromotive performance and mechanism in the levels of gene, cell and tissue will be investigated in this study.The project will also focus on the mulitple parameters, individualized manufacture methods and biomechanical properties of porous scaffolds using the low temperature rapid-prototyping technology. The phys-chemical properties, biocompatibility, and osteogenic potentials will al

英文关键词： cranial bone defect；scaffolds；3D printing；icaritin；osteogenic activity