TRAP1在赭曲霉毒素A干扰肾细胞凋亡与自噬内稳态中的作用机制

项目名称： TRAP1在赭曲霉毒素A干扰肾细胞凋亡与自噬内稳态中的作用机制

项目编号： No.31460426

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 谌小立

作者单位： 遵义医科大学

项目金额： 48万元

中文摘要： 赭曲霉毒素A(Ochratoxin A，OTA)由于其毒性高、普遍存在及不易降解，已成为食品安全重大风险诱因之一。前期研究表明，肿瘤坏死因子受体相关蛋白1（TRAP1）在OTA诱导的肾脏细胞毒性中起重要作用。本研究通过构建TRAP1干扰质粒，对人类胚肾293(HEK 293)细胞进行转染，然后采用qRT-PCR及Western Blot等实验方法，在TRAP1干扰及无干扰细胞系中研究OTA对肾细胞凋亡与自噬内稳态的影响，探索TRAP1在OTA干扰肾细胞凋亡与自噬内稳态中的作用机制，阐明TRAP1参与调控OTA诱导的肾细胞毒性作用机制。本项目的创新性在于首次深入研究TRAP1参与调控OTA诱导肾细胞毒性作用机制，为OTA毒性风险评估及预防治疗提供理论基础，进而保障粮食安全与人类健康。

中文关键词： 赭曲霉毒素A；肿瘤坏死因子受体相关蛋白1；细胞凋亡；自噬

英文摘要： Ochratoxin A (OTA) has become one of the significant food safety risk factors for its high toxicity, widespread existence and difficult degradation. Our previous results showed TRAP1 plays an important role in OTA-induced renal cytotoxicity. In the present study, Human Embryonic Kidney 293 cell (HEK 293) was transfected by plasmid with TRAP1 RNA interference (RNAi) effect. Research focused on the effect of Ochratoxin A on homeostasis between renal apoptosis and autophagy in TRAP1 RNAi and control cells though methods such as qRT-PCR and Western blot. Exploring the mechanism of Ochratoxin A-disturbed homeostasis between renal apoptosis and autophagy involved in TRAP1; clarifying OTA-induced renal cytotoxicity mechanism regulated by TRAP1. The innovation of this project lies in the fact that OTA-induced renal cytotoxicity mechanisms regulated by TRAP1 are researched for the first time. This study provides the theoretical foundation of OTA risk assessment and preventive treatment, and ensures food security and human health.

英文关键词： Ochratoxin A;TRAP1;apoptosis;autophagy