丁酸钠促进断奶仔猪肠道损伤修复的分子机制研究

项目名称： 丁酸钠促进断奶仔猪肠道损伤修复的分子机制研究

项目编号： No.31472101

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 畜牧学与草地科学

项目作者： 马曦

作者单位： 中国农业大学

项目金额： 88万元

中文摘要： 断奶仔猪腹泻严重影响养猪生产，近年研究发现断奶应激引起肠道损伤是其关键性因素。益生菌被证实可显著增加肠道丁酸含量，降低仔猪腹泻率，但机制不清。本课题组前期体外工作提示，丁酸可明显促进肠道上皮细胞损伤修复进程。为在动物水平验证效果，并深入阐明其机制，本项目拟以断乳仔猪为实验动物，在日粮中添加丁酸钠，观察其对仔猪腹泻的抑制效果；检测肠道黏膜屏障完整性，通过微生物组学和代谢组学分析，揭示丁酸对肠道微生物屏障形成的影响，特别是对肠道菌群结构和酶谱等的影响及调节模式；结合国际最新研究进展，建立体外肠道组织和细胞损伤修复模型，开展丁酸处理对肠道细胞的氧化应激、内源性抗菌肽表达，以及诱导损伤肠道上皮细胞重编程等生理进程的研究，并深入探索其调控方式和信号转导，最终阐明丁酸促进肠道损伤修复的分子机制。为通过营养手段改善仔猪在断奶应激时的肠道损伤修复进程，促进肠道屏障完善，减少断乳腹泻发生提供理论依据。

中文关键词： 断奶仔猪；丁酸钠；肠道损伤修复；微生物菌群；分子机制

英文摘要： Weaning diarrhea seriously affects the production of farming pig. Recent studies indicate that weaning stress-induced intestinal injury play a critical role in weaning diarrhea .Our study has showed that earlier administration of lactobacilli can significantly inhibit diarrhea,accompanied with significantly increasing the content of butyric acid in small intestine, but the mechanism is unclear. In vitro experiment showed that butyrate can significantly promote the wound healing process of intestinal epithelial cells. To further explore the molecular mechanisms and validated in animals, the study will select the weanning piglets as experimental animal, adding butyrate to feed and observing the effects of reducing diarrhea. The concentration of butyrate in intestine and its impact on the integrity of the intestinal mucosal barrier will be detected. By microbial genomics and metabolomics analysis, we hope to reveal the impact of butyrate on the intestinal microbial barrier formation, especially intestinal microflora of weaned piglets and zymography. Additionally, the mechanism of butyrate on the recovering of intestinal wound healing will be analysized, including reprogramming of intestinal epithelial cells and signal transduction. By means of inhibiting stress during the intestinal damage repair process of weaning piglets, the present study will provide a theoretical basis to reduce weaning diarrhea by nutritional methods.

英文关键词： Weaning piglets;Sodium butyrate;Intestinal wound healing;Microflora;Molecular mechanisms